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First Report of Meloidogyne enterolobii Causing Tuber Galling Damage on White Yam ( Dioscorea rotundata ) in Nigeria

2016; American Phytopathological Society; Volume: 100; Issue: 10 Linguagem: Inglês

10.1094/pdis-03-16-0348-pdn

1943-7692

Yao A. Kolombia, P. Lava Kumar, Abiodun O. Claudius-Cole, Gerrit Karssen, Nicole Viaene, Danny Coyne, Wim Bert,

Plant Pathogens and Resistance

HomePlant DiseaseVol. 100, No. 10First Report of Meloidogyne enterolobii Causing Tuber Galling Damage on White Yam (Dioscorea rotundata) in Nigeria PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Meloidogyne enterolobii Causing Tuber Galling Damage on White Yam (Dioscorea rotundata) in NigeriaY. A. Kolombia, P. Lava Kumar, A. O. Claudius-Cole, G. Karssen, N. Viaene, D. Coyne, and W. BertY. A. Kolombia, P. Lava Kumar, A. O. Claudius-Colehttp://orcid.org/0000-0003-1206-6325, G. Karssen, N. Viaene, D. Coyne, and W. BertAffiliationsAuthors and Affiliations Y. A. Kolombia P. Lava Kumar , International Institute of Tropical Agriculture (IITA), PMB 5320, Oyo Road, Ibadan, Nigeria A. O. Claudius-Cole , Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria G. Karssen , National Plant Protection Organization, 6706 EA Wageningen, The Netherlands N. Viaene , Institute for Agricultural and Fisheries Research (ILVO), 9820 Merelbeke, Belgium D. Coyne , IITA, Kasarani, P.O. Box 30772-00100, Nairobi, Kenya W. Bert , Nematology Research Unit, Department of Biology, Ghent University, 9000 Gent, Belgium. Published Online:10 Aug 2016https://doi.org/10.1094/PDIS-03-16-0348-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The white yam (Dioscorea rotundata) is the most widely grown species for food purposes in West Africa. Plant-parasitic nematode attack is among the major constraints to yam production (Bridge et al. 2005). Several species of nematodes are known to infect yams, including root-knot nematodes (RKN; Meloidogyne spp.), which cause tuber and root galling (Hunt and Handoo 2009). To date, M. incognita and M. javanica are reported in yam in Nigeria and elsewhere in Africa (Onkendi et al. 2014). During a taxonomic nematode survey, galled yam tubers were collected from markets and farmers' fields between November 2012 and February 2013 in Nigeria. Steam-sterilized soil planted with susceptible tomato (Solanum lycopersicum cv. Marmande) was infested with nematodes isolated from galled tubers and used for RKN population increase and species identification after maturation of nematodes. Ten young egg-laying females taken from these populations were used for species identification based on esterase and malate dehydrogenase isozymes patterns (Carneiro et al. 2000). The M2 and N1a isozyme electrophoresis phenotype identical to the M. enterolobii pattern described in Carneiro et al. (2000) was observed in 11% of 36 galled tubers examined. M. enterolobii was recovered from galled white yam in the Derived Savanna (Anambra State) and in the Southern Guinea Savanna (FCT Abuja and Niger States). Individual egg masses were removed and single-egg mass cultures were established on susceptible tomato to generate pure cultures of RKN. DNA isolated from a single juvenile of each of the 10 populations established was used for PCR amplification of mitochondrial COX2 using primer pair, COX2F (5′-TTGAATTTAAGTGTTGTTTATTAC-3′) and COX2R (5′-GATTAATACCACAAATCTCTGAAC-3′). The 432-bp PCR DNA product was sequenced on both strands (GenBank accession no. KU881046) and found to be 100% identical to M. enterolobii (KU881056, KP202351) (Janssen et al. 2016). To confirm M. enterolobii as a causal agent of the galling damage on white yam, egg masses removed from pure cultures were inoculated at a rate of 1 nematode/ml soil onto white yam cv. Makakusa planted in pots containing 6 liters of steam-sterilized top soil/white sand mixture (2:1 v/v) under screen house conditions. At harvest, 3 months after inoculation, roots and tubers exhibited severe galling symptoms typical of RKN. The nematode reproduction factor was on average 29 times the initial nematode density. The RKN, M. enterolobii, is therefore established as a new RKN species causing galling damage on yam. To our knowledge, this is the first record of M. enterolobii infecting yam and causing galling damage. This species is known to be present in West Africa and other continents affecting a range of crops, but not yam (Hunt and Handoo 2009). This extends the known host range for this damaging and highly aggressive nematode, and warrants consideration during resistance screening activities and other management strategies for yam.References:Bridge, J., et al. 2005. Page 221 in: Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. M. Luc, et al., eds. CAB International, Wallingford, U.K. https://doi.org/10.1079/9780851997278.0221 Crossref, Google ScholarCarneiro, R. M. D. G., et al. 2000. Nematol. 2:645. https://doi.org/10.1163/156854100509510 Crossref, ISI, Google ScholarHunt, D. J., and Handoo, Z. A. 2009. Page 55 in: Root-knot Nematodes. R. Perry, et al., eds. CAB International, Wallingford, U.K. https://doi.org/10.1079/9781845934927.0055 Crossref, Google ScholarJanssen, T., et al. 2016. Sci. Rep. 6:22591. https://doi.org/10.1038/srep22591 Crossref, ISI, Google ScholarOnkendi, E. M., et al. 2014. Plant Pathol. 63:727. https://doi.org/10.1111/ppa.12202 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 10 October 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 26 Sep 2016Published: 10 Aug 2016First Look: 6 Jun 2016Accepted: 1 Jun 2016 Page: 2173 Information© 2016 The American Phytopathological SocietyCited byMeloidogyne enterolobii risk to agriculture, its present status and future prospective for management24 January 2023 | Frontiers in Plant Science, Vol. 13Meloidogyne enterolobii : a threat to guava ( Psidium guajava ) cultivation22 October 2022 | Archives of Phytopathology and Plant Protection, Vol. 55, No. 17First Report of the Root-Knot Nematode Meloidogyne enterolobii Parasitizing Plantain (Musa spp., AAB) in NigeriaEmmanuel Olorunfemi Olajide, Yao Kolombia, Delphine Amah, Marjolein Couvreur, Rony Swennen, Daniel Leigh Coyne, Laura Cortada, and Wim Bert21 September 2022 | Plant Disease, Vol. 0, No. jaMeloidogyne enterolobii (Pacara earpod tree root-knot nematode)CABI Compendium, Vol. CABI CompendiumXanthosoma sagittifolium is resistant to Meloidogyne spp. and controls Meloidogyne enterolobii by soil biofumigation26 October 2020 | Journal of Nematology, Vol. 52, No. 1Morphological and molecular characterization of Pratylenchus species from Yam ( Dioscorea spp.) in West Africa22 January 2021 | Journal of Nematology, Vol. 52, No. 1Plant-Parasitic Nematodes and Food Security in Sub-Saharan AfricaAnnual Review of Phytopathology, Vol. 56, No. 1Diversity of Root-knot Nematodes Associated with Tubers of Yam ( Dioscorea spp.) Established Using Isozyme Analysis and Mitochondrial DNA-based Identification18 July 2017 | Journal of Nematology, Vol. 49, No. 2