Portal de Periódicos da CAPES

Autoradiographic Analysis of Alpha-Adrenoceptors and Muscarinic Cholinergic Receptors in the Hyperplastic Human Prostate

1989; Lippincott Williams & Wilkins; Volume: 142; Issue: 2 Part 1 Linguagem: Inglês

10.1016/s0022-5347(17)38780-3

1527-3792

Sarah‐Naomi James, Christopher R. Chapple, Mark Phillips, Pamela Greengrass, Michael Davey, Richard Turner-Warwick, E. J. G. MILROY, Geoffrey Burnstock,

Mass Spectrometry Techniques and Applications

No AccessJournal of Urology1 Aug 1989Autoradiographic Analysis of Alpha-Adrenoceptors and Muscarinic Cholinergic Receptors in the Hyperplastic Human Prostate S. James, C.R. Chapple, M.I. Phillips, P.M. Greengrass, M.J. Davey, R.T. Turner-Warwick, E.J.G. Milroy, and G. Burnstock S. JamesS. James , C.R. ChappleC.R. Chapple , M.I. PhillipsM.I. Phillips , P.M. GreengrassP.M. Greengrass , M.J. DaveyM.J. Davey , R.T. Turner-WarwickR.T. Turner-Warwick , E.J.G. MilroyE.J.G. Milroy , and G. BurnstockG. Burnstock View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)38780-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Radioligand receptor binding and autoradiography were used to characterize, localize and compare alpha-1 and alpha-2 adrenoceptors and muscarinic cholinergic receptor populations in human benign prostatic hyperplastic tissue. The binding of selective alpha-1 and alpha-2 ligands, [3H]- prazosin and [3H]-UK 14,304, to homogenates of human central and peripheral prostate was saturable and of high affinity. Scatchard analysis produced an equilibrium dissociation constant (KD) of 0.51 ± 0.10 nM for alpha-1 adrenoceptors, and 2.34 ± 0.40 nM for alpha-2 adrenoceptors. The mean densities, Bmax, of alpha-1 and alpha-2 adrenoceptors identified in the human adenomatous prostate were 65.9 ± 12.9 and 36.1 ± 7.0 fmoles/mg. protein respectively. Receptor autoradiography was used to examine the distribution of muscarinic cholinergic receptors ([3H]-QNB), alpha-1 adrenoceptors ([3H]-prazosin]), and alpha-2 adrenoceptors ([3H]-rauwolscine) on consecutive sections of benign hyperplastic prostatic tissue. Although both subtypes of adrenoceptor were seen in the stromal component of the hyperplastic prostate, there was a substantial predominance of alpha-1 adrenoceptors. A densitometric computer-assisted analysis was performed on the autoradiographic slides to determine the mean ratio of specific alpha-1: alpha-2 adrenoceptors in the stromal compartment of the hyperplastic tissue. The ratio, expressed as % grain occupancy/unit area, was 3.9 ± 0.75, which is in agreement with a functional alpha-1 adrenoceptor predominance shown in previous studies. Although sparsely distributed in the stroma, a dense alpha-2 adrenoceptor population was seen in association with blood vessels, and in close proximity to the base of some of the [3H]-QNB-labelled prostatic glandular epithelial cells. These results clearly illustrate a differential localization of specific receptors to precise prostatic regions, and future correlation between function and location might enable more selective therapeutic treatment of benign prostatic hyperplasia. © 1989 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByCHEN Q, TAKAHASHI S, ZHONG S, HOSODA C, ZHENG H, OGUSHI T, FUJIMURA T, OHTA N, TANOUE A, TSUJIMOTO G and KITAMURA T (2018) FUNCTION OF THE LOWER URINARY TRACT IN MICE LACKING α1d-ADRENOCEPTORJournal of Urology, VOL. 174, NO. 1, (370-374), Online publication date: 1-Jul-2005.Zlotta A, Raviv G, Peny M, Noel J, Haot J and Schulman C (2018) Possible Mechanisms of Action of Transurethral Needle Ablation of the Prostate on Benign Prostatic Hyperplasia Symptoms: A Neurohistochemical StudyJournal of Urology, VOL. 157, NO. 3, (894-899), Online publication date: 1-Mar-1997.Yazawa H, Saita Y, Iida E, Honma Y, Morita T and Honda K (2018) Characterization of Muscarinic Cholinoceptor in Primary Culture of Smooth Muscle Cells from Human ProstateJournal of Urology, VOL. 152, NO. 6 Part 1, (2173-2177), Online publication date: 1-Dec-1994.Yablonsky F, Savasta M, Feuerstein C and Poirier M (2018) Effects of Transection of the Spinal Cord in the Rat: Cystometric and Autoradiographic StudiesJournal of Urology, VOL. 152, NO. 4, (1315-1322), Online publication date: 1-Oct-1994.Price D, Schwinn D, Lomasney J, Allen L, Caron M and Lefkowitz R (2018) Identification, Quantification, and Localization of mRNA for Three Distinct Alpha1 Adrenergic Receptor Subtypes in Human ProstateJournal of Urology, VOL. 150, NO. 2 Part 1, (546-551), Online publication date: 1-Aug-1993.Fukumoto Y, Yoshida M, Dokita S, Kamai T, Weiss R and Latifpour J (2018) The Reversal Effect of Insulin on Diabetes-Induced Alterations In Beta Adrenergic and Muscarinic Receptors in Rat ProstateJournal of Urology, VOL. 149, NO. 6, (1602-1606), Online publication date: 1-Jun-1993.Kawabe K, Ueno A, Takimoto Y, Aso Y and Kato H (2018) Use of an α1-Blocker, YM617, in the Treatment of Benign Prostatic HypertrophyJournal of Urology, VOL. 144, NO. 4, (908-911), Online publication date: 1-Oct-1990.Kawabe K, Moriyama N, Hamada K and Ishima T (2018) Density and Localization of Alpha1-Adrenoceptors in Hypertrophied ProstateJournal of Urology, VOL. 143, NO. 3, (592-595), Online publication date: 1-Mar-1990. Volume 142Issue 2 Part 1August 1989Page: 438-444 Advertisement Copyright & Permissions© 1989 by The American Urological Association Education and Research, Inc.MetricsAuthor Information S. James More articles by this author C.R. Chapple More articles by this author M.I. Phillips More articles by this author P.M. Greengrass More articles by this author M.J. Davey More articles by this author R.T. Turner-Warwick More articles by this author E.J.G. Milroy More articles by this author G. Burnstock More articles by this author Expand All Advertisement PDF DownloadLoading ...