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BIBTEX RIS

Chimeric spider silk production in microalgae: a modular bionanomaterial

2016; Pensoft Publishers; Volume: 2; Linguagem: Inglês

10.3897/rio.2.e9342

2367-7163

João Vitor Dutra Molino, Tiago Lubiana, Livia Seno Ferreira‐Camargo, Miguel Croce, Allan Tanaka, Felipe Xavier Buson, Pedro Ribeiro, Antony Campos-Salazar, Eduardo Antonio, André Maizel, Viviane Siratuti, C. S. G. Costa, Samarina Rodrigues Wlodarczyk, Raquel de Souza Lima, Fabio Mello, Stephen P. Mayfield, João Carlos Monteiro de Carvalho,

Enzyme Production and Characterization

The recombinant proteins, spider silk proteins and enzybiotics, will be expressed in Chlamydomonas reinhardtii strains by nuclear transformation. Each recombinant strain will express a different protein, which will contain the N- and C-terminal polymerization domains from native spider silk proteins. These domains are essential to the polymerization step and, subsequently, for production of a material very similar to silk. This material will be evaluated regarding its antimicrobial and mechanical properties, as well as the system productivity. These results may shed some light on spider silk-based immobilization support effectiveness, even for other biotechnological applications, such as the one idealized here.