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Chimeric molecules with multiple neurotrophic activities reveal structural elements determining the specificities of NGF and BDNF.

1991; Springer Nature; Volume: 10; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07743.x

1460-2075

Carlos F. Ibáñez, Ted Ebendal, Håkan Persson,

14-3-3 protein interactions

Research Article1 August 1991free access Chimeric molecules with multiple neurotrophic activities reveal structural elements determining the specificities of NGF and BDNF. C.F. Ibáñez C.F. Ibáñez Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. Search for more papers by this author T. Ebendal T. Ebendal Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. Search for more papers by this author H. Persson H. Persson Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. Search for more papers by this author C.F. Ibáñez C.F. Ibáñez Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. Search for more papers by this author T. Ebendal T. Ebendal Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. Search for more papers by this author H. Persson H. Persson Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. Search for more papers by this author Author Information C.F. Ibáñez1, T. Ebendal1 and H. Persson1 1Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden. The EMBO Journal (1991)10:2105-2110https://doi.org/10.1002/j.1460-2075.1991.tb07743.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are two members of a family of neurotrophic factors which show both overlapping and distinct neurotrophic activities. Using site-directed mutagenesis, chimeric molecules were constructed where different combinations of sequences from BDNF replaced the corresponding sequences in NGF. The resulting molecules were transiently expressed in COS cells and conditioned media containing the chimeric proteins were assayed for biological activity in explanted chick sympathetic, spinal and nodose ganglia. Our results show that the biological specificities of the two proteins are obtained by specific combinations of a set of sequences that differ between the two molecules. Some of these combinations allowed us to engineer molecules which display multiple neurotrophic activities recruited from both the NGF and BDNF proteins. Previous ArticleNext Article Volume 10Issue 81 August 1991In this issue RelatedDetailsLoading ...