Artigo Acesso aberto

The Location of the Donnan Free Space in Disks of Beetroot Tissue

1965; CSIRO Publishing; Volume: 18; Issue: 3 Linguagem: Inglês

10.1071/bi9650547

ISSN

0004-9417

Autores

M. G. Pitman,

Tópico(s)

Marine Biology and Environmental Chemistry

Resumo

This paper describes experiments which show that the cell walls of beetroot tissue contain sufficient cation�exchange sites to account for at least 95% of the Donnan free space (D.F.S.) as measured by Briggs, Hope, and Pitman (1958). The contribution of the cytoplasm to the D.F.S. in their measurements was therefore less than 5%. The exchange sites in the D.F.S. of the tissue and in the cell walls have the same pKa of about 2�8, and are considered to be due to bound Ilronic acids.

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