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Capillary Zone Electrophoretic Determination of Histamine in Fish

1994; Oxford University Press; Volume: 77; Issue: 4 Linguagem: Inglês

10.1093/jaoac/77.4.881

1944-7922

Barry Mopper, Carl J Sciacchttano,

Advanced biosensing and bioanalysis techniques

Histamine, the principal causative agent in scombroid food poisoning, was analyzed in seafood by a new, rapid, and sensitive method using capillary zone electrophoresis (CZE) with UV detection at 210 nm. Incurred histamine in methanolic fish extracts migrated within 4 min in a fused silica capillary filled with 0.02 M citrate buffer, pH 2.5, under an applied potential of 375 V/cm. The analytical response was linear from 0.5 to 100 ppm histamine (correlation coefficient, r = 0.999). The coefficients of variation for migration time and peak area response were < 1 and < 3%, respectively. Recovery of histamine in fortified fish composites was satisfactory. CZE was considered for alternative application in seafood speciation.