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A Portable Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella typhimurium

2016; Korean Vacuum Society; Volume: 25; Issue: 3 Linguagem: Inglês

10.5757/asct.2016.25.3.61

2288-6559

Hoang Hiep Nguyen, So Yeon Yi, Woubit Abdela, Moonil Kim,

Advanced Biosensing Techniques and Applications

Here, the rapid detection of Salmonella typhimurium by a portable surface plasmon resonance (SPR) biosensor in which the beam from a diode laser is modulated by a rotating mirror is reported. Using this system, immunoassay based on lipopolysaccharides (LPS)-specific monoclonal anti-Salmonella antibody was performed. For the purpose of orientation-controlled immobilization of antibodies on the SPR chip surface, the cysteine-mediated immobilization method, which is based on interaction between a gold surface and a thiol group (-SH) of cysteine, was adopted. As a result, using the portable SPR-based immunoassay, we detected S. typhimurium in the range from 10^7 CFU/mL to 10^9 CFU/mL within 1 hour. The results indicate that the portable SPR system could be potentially applied for general laboratory detection as well as on-site monitoring of foodborne, clinical, and environmental agents of interest.