Chromatographic Separation of Aldosterone and Its Metabolites
2021; Informa; Linguagem: Inglês
10.1201/9781003209690-6
AutoresDavid J. Morris, Ritsuko Tsai,
Tópico(s)Hormonal and reproductive studies
ResumoExcessive secretion of several of the nonaldosterone adrenocortical steroids has been reported in patients with hypertension, particularly those with low renin essential hypertension. Higher levels of several of these steroid hormone metabolites have been correlated with the development of hypertension both in experimental animals and in humans. The mineralocorticoid aldosterone plays an important role in the kidney by regulating active sodium transport (antinatriuresis) and potassium excretion (kaliuresis), and hence fluid balance. High-Pressure Liquid Chromatography (HPLC) was then investigated to develop a rapid fingerprinting system for the separation and isolation of the reduced and polar neutral metabolites of aldosterone in these tissues. These metabolites possess a wide range of polarities. An Improved HPLC system with reproducible and good resolution of the polar neutral metabolites of aldosterone also has been developed, employing either a gradient of aqueous acetonitrile or a stepwise elution with various percentages of aqueous acetonitrile.
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