Changes in the Chemical Barrier Composition of Tears in Alzheimer’s Disease Reveal Potential Tear Diagnostic Biomarkers
2016; Public Library of Science; Volume: 11; Issue: 6 Linguagem: Inglês
10.1371/journal.pone.0158000
ISSN1932-6203
AutoresGergő Kalló, Miklós Emri, Zsófia Varga, Bernadett Ujhelyi, József Tőzsér, Adrienne Csutak, Éva Csősz,
Tópico(s)Glaucoma and retinal disorders
ResumoAlzheimer's disease (AD) is one of the most common neurodegenerative diseases, with increasing prevalence affecting millions of people worldwide. Currently, only autopsy is able to confirm the diagnosis with a 100% certainty, therefore, biomarkers from body fluids obtained by non-invasive means provide an attractive alternative for the diagnosis of Alzheimer`s disease. Global changes of the protein profile were examined by quantitative proteomics; firstly, electrophoresis and LC-MS/MS were used, thereafter, SRM-based targeted proteomics method was developed and applied to examine quantitative changes of tear proteins. Alterations in the tear flow rate, total tear protein concentration and composition of the chemical barrier specific to AD were demonstrated, and the combination of lipocalin-1, dermcidin, lysozyme-C and lacritin was shown to be a potential biomarker, with an 81% sensitivity and 77% specificity.
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