Artigo Acesso aberto

Development of Real-Time PCR Assays for Detecting Matrix Metalloproteinases-2 & 9 Over-expression in Canine Mammary Tumours

2016; Nexus Academic Publishers; Volume: 4; Issue: 7 Linguagem: Inglês

10.14737/journal.aavs/2016/4.7.342.345

ISSN

2309-3331

Autores

Sonal Saxena, Sameer Shrivastava, Richa Arora, Shahid Hussain, Subas Chandra Jena, Manoj Kumar, Resma Kochumanayin Vasu, Saumya Srivastava, Priyanka Sharma, Naveen Kumar, Kuldeep Dhama,

Tópico(s)

Cell Adhesion Molecules Research

Resumo

M atrix metalloproteinases (MMPs) encompasses a family of secreted, calcium dependent, zinc containing endopeptidases.These enzymes are responsible for tissue remodelling and are capable of degrading all kinds of extracellular matrix proteins including collagens, matrix glycoproteins, gelatin, elastins, and proteoglycans.MMPs are involved in regulation of cell behaviours like cellular differentiation, proliferation, angiogenesis, invasion, and apoptosis.Thus, these enzymes have multi-factorial roles during tumor progression: promoting establishment, tumor cell exfoliation, invasion and angiogenesis.The degra-dation of extracellular matrix components is crucial for tumor cell invasion and MMPs, especially MMP2 (72 kDa gelatinase A) and MMP9 (92 kDa gelatinase B), play critical role in degradation of gelatin and type IV collagen, the two major constituents of extracellular matrix.MMP9 and MMP2 are involved in invasion and metastases of many human cancers such as cancers of colon (Karakiulakis et al.,

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