Portal de Periódicos da CAPES

Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain

2016; Nature Portfolio; Volume: 34; Issue: 8 Linguagem: Inglês

10.1038/nbt.3594

1546-1696

Jeffrey N. Stirman, Ikuko T. Smith, Michael W. Kudenov, Spencer L. Smith,

Cell Image Analysis Techniques

Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.