First Report of Dickeya dianthicola Causing Blackleg and Bacterial Soft Rot on Potato in Maine
2016; American Phytopathological Society; Volume: 100; Issue: 11 Linguagem: Inglês
10.1094/pdis-12-15-1513-pdn
ISSN1943-7692
AutoresHe Jiang, Jianjun Hao, Stephen B. Johnson, Robert Brueggeman, Gary A. Secor,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 100, No. 11First Report of Dickeya dianthicola Causing Blackleg and Bacterial Soft Rot on Potato in Maine PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Dickeya dianthicola Causing Blackleg and Bacterial Soft Rot on Potato in MaineH. H. Jiang, J. J. Hao, S. B. Johnson, R. S. Brueggeman, and G. SecorH. H. Jiang, J. J. Hao, S. B. Johnson, R. S. Brueggeman, and G. SecorAffiliationsAuthors and Affiliations H. H. Jiang J. J. Hao , School of Food and Agriculture, University of Maine, Orono, ME 04469 S. B. Johnson , University of Maine Cooperative Extension, Presque Isle, ME 04769 R. S. Brueggeman G. Secor , Department of Plant Pathology, North Dakota State University, Fargo, ND 58108. Published Online:18 Aug 2016https://doi.org/10.1094/PDIS-12-15-1513-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat An outbreak of blackleg on potato (Solanum tuberosum) occurred in Maine in the summer of 2015. This disease impacted potato production across the state as well as other northeastern and mid-Atlantic states where Maine potato seeds were shipped. The disease is commonly caused by Pectobacterium spp. (Ma et al. 2007), but the aggressiveness of this disease outbreak led to the reevaluation of the pathogen taxa. Based on the field survey of 'Reba' and other cultivars, potato plants with typical blackleg symptoms, including darkened and necrotic basal stems, wilting canopy, and rot-tuber-caused nonemergence, were collected from more than 10 different farms in Maine. Basal stems (2 cm in length) of symptomatic potato plants were cut and surface disinfested with 75% ethanol for 40 s and then with 0.65% sodium hypochlorite for 1 min, followed by rinsing with sterile distilled water. The stem segment was further chopped into small pieces in a sterile container with sterile water added (700 µl per segment) for 5 min to allow the bacteria to release from the stem tissue. The bacterial suspension was plated on crystal violet polypectate agar (Hélias et al. 2012), a semiselective medium for pectolytic bacteria. Typical bacterial colonies were isolated. Bacterial DNA was extracted from the pure culture of strain ME30 using the Qiagen DNeasy Kit. PCR was conducted using Dickeya genus-specific primers, ADE-f/ADE-r primers (Nassar et al. 1996). Isolates resulting in the expected 520 bp band were further analyzed. Utilizing all possible Dickeya species submitted to the NCBI database, specific primers were designed from conserved regions of the flagellin (FliC) gene from D. dianthicola, D. dadanti, and D. solani. The primers were compared by BLASTn analysis to confirm that they would not cross amplify from any other known bacterial species submitted to GenBank. The Dickeya FliC gene-specific amplicons were generated from genomic DNA isolated from single colonies, barcoded with Ion Torrent adapters and sequenced on an Ion Torrent 314 microprocessor sequencing chip. The isolate ME30 provided 23,001 sequences (GenBank accession KX238901), which were aligned in CLCBio genomics workbench showing homogeneity of the FliC gene amplicon. Alignment of the amplicon sequence with the D. dianthicola strain LMG 2485 sequence present in the NCBI database (JN617628.1) produced 100% nucleic acid identity. Thus, the isolate was indicated to be D. dianthicola. To examine the pathogenicity of the bacterial isolates, a bacterial suspension of each Dickeya-positive isolate was inoculated on potato (cv. Shepody) plants in a greenhouse. Potato tubers were planted in 1-gallon pots containing potting mix with four replicates. Three weeks after plant emergence, 10 µl of cell suspension (107 cfu/ml) of strain ME30 was injected into the lower part of potato stem with a syringe, and sterile water was used for control. Sixteen days after inoculation, the pathogen-inoculated stems showed black colored and rotten stems, but the control plants stayed symptomless. Bacteria were isolated from the lesion and confirmed to be the same organism as the inoculum using the molecular techniques previously described. This is the first report in Maine that D. dianthicola causes blackleg of potato, which has not been reported on other crops either.References:Hélias, V., et al. 2012. Plant Pathol. 61:339. https://doi.org/10.1111/j.1365-3059.2011.02508.x Crossref, ISI, Google ScholarMa, B., et al. 2007. Phytopathology 97:1150. https://doi.org/10.1094/PHYTO-97-9-1150 Link, ISI, Google ScholarNassar, A., et al. 1996. Appl. Environ. Microbiol. 62:2228. Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 11 November 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 7 Oct 2016Published: 18 Aug 2016First Look: 8 Jul 2016Accepted: 12 Jun 2016 Pages: 2320-2320 Information© 2016 The American Phytopathological SocietyCited byMapping the Complex Transcriptional Landscape of the Phytopathogenic Bacterium Dickeya dadantiimBio, Vol. 13, No. 3Pangenomic Analysis of Dickeya dianthicola Strains Related to the Outbreak of Blackleg and Soft Rot of Potato in the United StatesTongling Ge, He Jiang, Ek Han Tan, Steven B. Johnson, Robert P. Larkin, Amy O. 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