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Nanostructured Aptamer-Functionalized Black Phosphorus Sensing Platform for Label-Free Detection of Myoglobin, a Cardiovascular Disease Biomarker

2016; American Chemical Society; Volume: 8; Issue: 35 Linguagem: Inglês

10.1021/acsami.6b06488

1944-8252

Vinod Kumar, Jack R. Brent, Munish Shorie, Harmanjit Kaur, Gaganpreet Chadha, Andrew G. Thomas, Edward A. Lewis, Aidan P. Rooney, Lan Nguyen, Xiangli Zhong, M.G. Burke, Sarah J. Haigh, Alex S. Walton, Paul D. McNaughter, Aleksander A. Tedstone, Nicky Savjani, C.A. Muryn, Paul O’Brien, Ashok K. Ganguli, David J. Lewis, Priyanka Sabherwal,

Conducting polymers and applications

We report the electrochemical detection of the redox active cardiac biomarker myoglobin (Mb) using aptamer-functionalized black phosphorus nanostructured electrodes by measuring direct electron transfer. The as-synthesized few-layer black phosphorus nanosheets have been functionalized with poly-l-lysine (PLL) to facilitate binding with generated anti-Mb DNA aptamers on nanostructured electrodes. This aptasensor platform has a record-low detection limit (∼0.524 pg mL–1) and sensitivity (36 μA pg–1 mL cm–2) toward Mb with a dynamic response range from 1 pg mL–1 to 16 μg mL–1 for Mb in serum samples. This strategy opens up avenues to bedside technologies for multiplexed diagnosis of cardiovascular diseases in complex human samples.