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Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton

2012; Humana Press; Linguagem: Inglês

10.1007/978-1-62703-266-7_15

1940-6045

Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, Hauke Werner,

Ubiquitin and proteasome pathways

Myelin, the multilayered membrane that enwraps and insulates neuronal axons for fast signal propagation, is a plasma membrane specialization of oligodendrocytes and Schwann cells in the central and peripheral nervous system, respectively. Here we provide our lab protocols for the purification of myelin from mouse brains and for gel-based and gel-free proteomic applications, as well as a brief discussion with respect to our current knowledge of the myelin cytoskeleton.