Portal de Periódicos da CAPES

The Fungal Endophyte Nigrospora oryzae Produces Quercetin Monoglycosides Previously Known Only from Plants

2016; Wiley; Volume: 1; Issue: 11 Linguagem: Inglês

10.1002/slct.201600478

2365-6549

Sherif S. Ebada, Peter M. Eze, Festus Basden Chiedu Okoye, Charles Okechukwu Esimone, Peter Proksch,

Phytochemistry and Biological Activities

ChemistrySelectVolume 1, Issue 11 p. 2767-2771 Full Paper The Fungal Endophyte Nigrospora oryzae Produces Quercetin Monoglycosides Previously Known Only from Plants Dr. Sherif S. Ebada , Corresponding Author Dr. Sherif S. Ebada sherif_elsayed@pharma.asu.edu.eg Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, D-40225 Düsseldorf, Germany Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Ain-Shams University, 11566 Cairo, EgyptSearch for more papers by this authorPeter Eze , Peter Eze Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, D-40225 Düsseldorf, Germany Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaSearch for more papers by this authorDr. Festus B. C. Okoye, Dr. Festus B. C. Okoye Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaSearch for more papers by this authorProf. Charles O. Esimone, Prof. Charles O. Esimone Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaSearch for more papers by this authorProf. Peter Proksch, Corresponding Author Prof. Peter Proksch proksch@uni-duesseldorf.de Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, D-40225 Düsseldorf, GermanySearch for more papers by this author Dr. Sherif S. Ebada , Corresponding Author Dr. Sherif S. Ebada sherif_elsayed@pharma.asu.edu.eg Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, D-40225 Düsseldorf, Germany Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Ain-Shams University, 11566 Cairo, EgyptSearch for more papers by this authorPeter Eze , Peter Eze Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, D-40225 Düsseldorf, Germany Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaSearch for more papers by this authorDr. Festus B. C. Okoye, Dr. Festus B. C. Okoye Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaSearch for more papers by this authorProf. Charles O. Esimone, Prof. Charles O. Esimone Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, NigeriaSearch for more papers by this authorProf. Peter Proksch, Corresponding Author Prof. Peter Proksch proksch@uni-duesseldorf.de Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine Universität, D-40225 Düsseldorf, GermanySearch for more papers by this author First published: 22 July 2016 https://doi.org/10.1002/slct.201600478Citations: 15Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Three quercetin-3-O-glycosides (1–3) were unequivocally identified from Nigrospora oryzae, an endophyte isolated from leaves of the Nigerian mistletoe Loranthus micranthus. The presence of flavonoid glycosides in a fungal endophyte is remarkable, unprecedentedly reported and confirmed here for the first time adding further evidence to the remarkable plasticity of fungal secondary metabolism. Abstract Three quercetin-3-O-glycosides (1–3) were obtained from the endophytic fungus Nigrospora oryzae isolated from leaves of the Nigerian mistletoe Loranthus micranthus. The compounds were unequivocally identified based on their one and two dimensional NMR spectra and on their mass spectra. A fourth flavonoid glycoside (4) was tentatively identified as a luteolin monoglycoside. Comparative HPLC analyses of extracts of the endophyte and of the host plant indicated that compounds (1–3) were present as minor constituents in the extract of the host plant Loranthus micranthus whereas the latter was dominated by different gallic acid and by catechin derivatives that were missing in the endophyte extract. The presence of flavonoid glycosides in a fungal endophyte is remarkable and is unequivocally confirmed here for the first time. So far, compounds (1–3) were only known as commonly occurring plant secondary metabolites. This study adds further evidence to the remarkable plasticity of fungal secondary metabolism which has repeatedly been shown to produce the same compounds as present in plants. Citing Literature Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description slct201600478-sup-0001-misc_information.pdf2.5 MB Supplementary Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume1, Issue11July 16, 2016Pages 2767-2771 RelatedInformation