Solid-Phase Fluorescence Immunoassay for the Detection of Antibiotic Residues in Milk
1996; American Chemical Society; Linguagem: Inglês
10.1021/bk-1996-0621.ch036
ISSN1947-5918
AutoresAmit Kumar, Richard M. Rocco, Danton K. Leung, Larry Jang, Shanta Kharadia, Caroline Yu, Krista K. Hara-Mikami, Gwendolyn Μ. Jang, Marcos Piani,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoWe describe a rapid, inhibition immunoassay for several antibiotics found as residues in milk. The fluorophore is the recently developed compound, Cy-5™, and the excitation source is a semiconductor-diode laser. The solid-phase is the borosilicate glass surface (SiO2) of a capillary tube that has been treated with a silanizing reagent. β-Lactam antibiotics ( penicillin-G, ampicillin, cloxacillin, amoxicillin, ceftiofur and cephapirin) at the parts per billion level can be detected in a total assay time of less than three minutes. These assays were optimized to provide the greatest sensitivity at or near the regulatory safe/tolerance levels for the appropriate antibiotics. The potential for the use of this system for other types of assays will also be discussed.
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