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α-Phellandrene, a cyclic monoterpene, attenuates inflammatory response through neutrophil migration inhibition and mast cell degranulation

2016; Elsevier BV; Volume: 160; Linguagem: Inglês

10.1016/j.lfs.2016.07.008

1879-0631

Halmisson D'Arley S. Siqueira, Benedito S. Neto, Damião Pergentino de Sousa, Bruno da Silva Gomes, Francilene Vieira da Silva Freitas, Francisco Valmor Macedo Cunha, Carlos W. Wanderley, Gabriel Pinheiro, André George Ferreira Cândido, Deysi Viviana Tenazoa Wong, Ronaldo A. Ribeiro, Roberto César Pereira Lima‐Júnior, Francisco de Assis Oliveira,

Phytochemistry and Biological Activities

We aimed to investigate the modulating effect of α-phellandrene on neutrophil migration and mast cell degranulation processes.Male Wistar rats or Swiss mice were treated p.o. with vehicle (3% Tween 80, p.o.), α-phellandrene (50, 100, or 200mg/kg, p.o.), or dexamethasone (0.5mg/kg, p.o.) 1h before carrageenan injection. Then, the neutrophil migration in 6-day-old air pouches or peritoneal cavities. The leukocyte rolling and adhesion were measured in real time and assessed by intravital microscopy. ELISA was used to detect TNF-α and IL-6 in peritoneal lavage. Compound 48/80-induced mast cell degranulation was assessed in mesenteric rat tissues.In all the tested doses, α-phellandrene prevented carrageenan-induced neutrophil accumulation (P<0.05). As detected by intravital microscopy, α-phellandrene also inhibited leukocyte rolling and adhesion, as well as significantly inhibited the production of the pro-inflammatory cytokines TNF-α and IL-6. Moreover, the degranulation of compound 48/80-induced mast cells was also inhibited by α-phellandrene (P<0.001).These results suggest that α-phellandrene plays an important role as an anti-inflammatory agent through neutrophil migration modulation and mast cell stabilization.