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µ 1 -Opioid receptors in the dorsomedial and ventrolateral columns of the periaqueductal grey matter are critical for the enhancement of post-ictal antinociception
2016; Wiley; Volume: 70; Issue: 12 Linguagem: Inglês
10.1002/syn.21926
ISSN1098-2396
AutoresRenato Leonardo de Freitas, Priscila Medeiros, Asmat Ullah Khan, Norberto Cysne Coimbra,
Tópico(s)Neurotransmitter Receptor Influence on Behavior
ResumoSynapseVolume 70, Issue 12 p. 519-530 Research Article µ1-Opioid receptors in the dorsomedial and ventrolateral columns of the periaqueductal grey matter are critical for the enhancement of post-ictal antinociception Renato Leonardo de Freitas, Renato Leonardo de Freitas Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Department of Surgery and Anatomy, Multiuser Centre of Neurophysiology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Laboratory of Pain and Emotions, Department of Surgery and Anatomy, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Behavioural Neurosciences Institute, Av. do Café, 2450, Ribeirão Preto, São Paulo, 14050-220 BrazilSearch for more papers by this authorPriscila Medeiros, Priscila Medeiros Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Laboratory of Pain and Emotions, Department of Surgery and Anatomy, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 BrazilSearch for more papers by this authorAsmat Ullah Khan, Asmat Ullah Khan Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Department of Eastern Medicine and Surgery, School of Medical and Health Sciences of the University of Poonch Rawalakot, Azad Jammu and Kashmir, PakistanSearch for more papers by this authorNorberto Cysne Coimbra, Corresponding Author Norberto Cysne Coimbra nccoimbr@fmrp.usp.br Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Laboratory of Pain and Emotions, Department of Surgery and Anatomy, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Behavioural Neurosciences Institute, Av. do Café, 2450, Ribeirão Preto, São Paulo, 14050-220 BrazilCorrespondence Norberto Cysne Coimbra, Laboratório de Neuroanatomia & Neuropsicobiologia, Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (FMRP-USP), Av. dos Bandeirantes, 3900, Ribeirão Preto (SP) 14049-900, Brasil. Email:nccoimbr@fmrp.usp.brSearch for more papers by this author Renato Leonardo de Freitas, Renato Leonardo de Freitas Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Department of Surgery and Anatomy, Multiuser Centre of Neurophysiology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Laboratory of Pain and Emotions, Department of Surgery and Anatomy, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Behavioural Neurosciences Institute, Av. do Café, 2450, Ribeirão Preto, São Paulo, 14050-220 BrazilSearch for more papers by this authorPriscila Medeiros, Priscila Medeiros Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Laboratory of Pain and Emotions, Department of Surgery and Anatomy, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 BrazilSearch for more papers by this authorAsmat Ullah Khan, Asmat Ullah Khan Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Department of Eastern Medicine and Surgery, School of Medical and Health Sciences of the University of Poonch Rawalakot, Azad Jammu and Kashmir, PakistanSearch for more papers by this authorNorberto Cysne Coimbra, Corresponding Author Norberto Cysne Coimbra nccoimbr@fmrp.usp.br Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Laboratory of Pain and Emotions, Department of Surgery and Anatomy, Ribeirão Preto Medical School of the University of São Paulo (USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900 Brazil Behavioural Neurosciences Institute, Av. do Café, 2450, Ribeirão Preto, São Paulo, 14050-220 BrazilCorrespondence Norberto Cysne Coimbra, Laboratório de Neuroanatomia & Neuropsicobiologia, Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (FMRP-USP), Av. dos Bandeirantes, 3900, Ribeirão Preto (SP) 14049-900, Brasil. Email:nccoimbr@fmrp.usp.brSearch for more papers by this author First published: 09 August 2016 https://doi.org/10.1002/syn.21926Citations: 11Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Generalised tonic and tonic–clonic seizures are followed by significant increase in nociceptive thresholds in both laboratory animals and humans. The endogenous opioid peptides play a role in antinociceptive signalling, and the periaqueductal grey matter (PAG) is recruited to induce analgesia. Thus, the aim of this investigation was to evaluate the role of µ1-opioid receptors in the dorsomedial (dm) and ventrolateral (vl) columns of PAG in post-ictal antinociception. Pentylenetetrazole (PTZ; 64 mg/kg), which is an ionotropic GABA-mediated Cl− influx antagonist, was intraperitoneally (IP) administered to induce tonic–clonic seizures in Wistar rats. The tail-flick test was used to measure the nociceptive threshold. Microinjections of naltrexone (5.0 µg/0.2 µL), which is a non-selective opioid receptor antagonist, in both dmPAG and vlPAG decreased the tonic–clonic seizure-induced antinociception in seizing animals from 10 to 120 min after seizures. Furthermore, microinjections of the µ1-opioid receptor-selective antagonist naloxonazine (5.0 µg/0.2 µL) into the dmPAG decreased post-ictal antinociception immediately after convulsive reactions and from 10 to 90 min after seizures. However, vlPAG-pretreatment with naloxonazine at the same concentration decreased the post-ictal antinociception 30 min after the onset of tonic–clonic seizures and the nociceptive threshold returned to basal values 120 min after seizures. These findings indicate that µ1-opioid receptor-signalling mechanisms in both dmPAG and vlPAG play a relevant role in the organisation of post-ictal antinociception. In addition, µ1-opioid receptors in the dmPAG rather than in vlPAG seem to be more critically recruited during the antinociception induced by generalised tonic–clonic seizures. Citing Literature Volume70, Issue12December 2016Pages 519-530 RelatedInformation
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