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DNA Microchip Technology in the Plant Tissue Culture Industry

2002; American Chemical Society; Linguagem: Inglês

10.1021/bk-2002-0829.ch008

1947-5918

K. Kunert, Juan Vorster, C. Bester, Christopher A. Cullis,

Banana Cultivation and Research

Any company involved in micropropagation of plants must be able to demonstrate that the plants produced remain true-to-type as an important part of quality assurance. Modern approaches to detect undesired plant off-types in the in vitro propagation process might also include the application of the "DNA-microchip" technology using DNA microarrays carrying hybridization targets isolated from undesirable plant variants. Representational Difference Analysis (RDA) has been investigated as a technique for the identification and isolation of potential hybridization targets in a pilot study of somatic embryogenesis in date palm. RDA is a subtractive DNA technique allowing a significant fraction of the plant genome (up to 15%) to be compared between closely related plant lines and to isolate DNA differences between two types of plants. Three difference products from tissue culture-derived plants were isolated by RDA. One product has been further characterized for its potential to monitor genetic variation during the micropropagation process.