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Significance of autoclaving-induced toxicity from and hydrolysis of carbohydrates in in vitro studies of pollen germination and tube growth

1989; Elsevier BV; Volume: 55; Issue: 1 Linguagem: Inglês

10.1016/s0254-6299(16)31225-x

1727-9321

J.H. de Lange,

Plant tissue culture and regeneration

Autoclaving of various carbohydrates resulted in decreased in vitro pollen germination and/or pollen tube growth in species belonging to different families. This toxicity was eliminated by treatment of autoclaved sucrose and glucose solutions with activated charcoal. Autoclaving-induced hydrolysis of sucrose at pH 6 resulted in stimulated Agapanthus praecox pollen growth (in detoxified media) probably due to the formation of glucose which was found to be a superior carbohydrate source for in vitro pollen growth in this species. Considering these results as well as previous reports on anther culture studies, autoclaving-induced toxicity from and hydrolysis of carbohydrates should be taken into account in all pollen and anther culture studies as well as in vitro pollination of ovules. Outoklavering van verskeie koolhidrate het aanleiding gegee tot verminderde in vitro-stuifmeelontkieming en/of stuifmeelbuisgroei in spesies behorende tot verskillende families. Outoklavering-geïnduseerde hidrolise van sukrose by pH 6 het verbeterde Agapanthus praecox-stuifmeelgroei tot gevolg gehad (in gedetoksifeerde mediums) waarskynlik a.g.v. die vrystelling van glukose wat ’n hoogs-effektiewe koolhidraatbron vir in vitro-stuifmeelgroei in die betrokke spesie is. Op grond van hierdie resultate sowel as dié van vroeëre ondersoeke van helmknopkulture, behoort outoklavering-geïnduseerde toksisiteit en hidrolise van koolhidrate in ag geneem te word by alle stuifmeel- en helmknopkulture en by in vitro-bestuiwing van saadknoppe.