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Production of 8,11,14,17‐ cis ‐eicosatetraenoic acid (20:4ω‐3) by a Δ5 and Δ12 desaturase‐defective mutant of an arachidonic acid‐producing fungus Mortierella alpina 1S‐4

1998; Wiley; Volume: 75; Issue: 11 Linguagem: Inglês

10.1007/s11746-998-0084-x

1558-9331

Hiroshi Kawashima, Eiji Sakuradani, Nozomu Kamada, Kengo Akimoto, Kyoko Konishi, Jun Ogawa, Sakayu Shimizu,

Enzyme Catalysis and Immobilization

Abstract A Δ5 and Δ12 desaturase‐defective mutant of an arachidonic acid‐producing fungus, Mortierella alpina 1S‐4, produced 8,11,14,17‐ cis ‐eicosatetraenoic acid (20:4ω3) intracellularly when grown with linseed oil. Dihomo‐γ‐linolenic acid was the only C 20 polyunsaturated fatty acid (4.9 wt% of total mycelial fatty acids) other than 20:4ω3. AA and 5,8,11,14,17‐ cis ‐eicosapentaenoic acid were not detected. The mycelial lipids consisted of 82.2% (by mol) triacylglycerol (TG), 7.1% diacylglycerol, 8.9% phospholipids (PL), and 1.9% free fatty acids. The percentage of 20:4ω3 was higher in PL (30.1%) than in TG (11.6%), and highest in phosphatidylcholine (38.9%). Under the optimal conditions with a 5‐L jar fermenter, 20:4ω3 production amounted to 97.4 mg/g dry mycelia with a mycelial yield of 23 g/L on the twelfth day (corresponding to 2.24 g/L medium and 37.1% of total mycelial fatty acids).