Formation of β,γ-methylene-7,8-dihydroneopterin 3′-triphosphate from β,γ-methyleneguanosine 5′-triphosphate by GTP cyclohydrolase I of Escherichia coli
1984; Elsevier BV; Volume: 233; Issue: 2 Linguagem: Inglês
10.1016/0003-9861(84)90470-3
ISSN1096-0384
AutoresJuan Ferré, K. Bruce Jacobson,
Tópico(s)Amino Acid Enzymes and Metabolism
ResumoGTP cyclohydrolase I of Escherichia coli converts [β,γ-methylene]GTP to a fluorescent product that is characterized as [β,γ-methylene]dihydroneopterin triphosphate. Interaction between the GTP analog and the enzyme gave a Ki of 3.0 μm, which may be compared to the Km of 0.1 μm for GTP. This new analog of dihydroneopterin triphosphate may, in turn, be converted to the same greenish-yellow pteridines (compounds X, X1, and X2) that are obtained from dihydroneopterin triphosphate. Because of its stability to phosphatase action, this analog may be useful for studies in pteridine metabolism.
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