Biology of the Filamentous Bacteriophage
1996; Elsevier BV; Linguagem: Inglês
10.1016/b978-012402380-2/50003-4
Autores Tópico(s)RNA and protein synthesis mechanisms
ResumoThis chapter describes various aspects of the biology of the filamentous bacteriophage. A number of filamentous phages have been identified which are able to infect a variety of gram negative bacteria. They have a single-stranded, covalently closed DNA genome which is encased in a long cylinder approximately 7 nm wide and by 900 to 2000 nm in length. The genome of the Ff phage is a single-stranded covalently closed DNA molecule. Dimers of pV bind single-strand DNA in a highly cooperative manner. It is this binding that prevents the conversion of the newly synthesized single-stranded DNA. The products of the next group of genes, III, VI, VII, VIII, and IX, constitute the capsid proteins of the phage particle. All reside in the cytoplasmic membrane until they are assembled into a phage particle. The product of gene IV is synthesized with a 21-residue amino-terminal signal sequence. It is secreted across the cytoplasmic membrane in a manner dependent on the Sec system of the host. Polyphage produced in the absence of pVI is very unstable compared to polyphage produced in the absence of pill. This observation suggests that the addition of pVI stabilizes the phage structure and provides a structure for the binding of pIII.
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