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Enzyme-Based Electrochemical Biosensor with DNA Array Chip

2000; Linguagem: Inglês

10.1007/978-94-017-2264-3_119

1556-5890

Jen-Jr Gau, Esther H. Lan, Bruce Dunn, Chih‐Ming Ho,

Molecular Junctions and Nanostructures

A reusable DNA sensor array# for rapid biological agent detection has been fabricated on a silicon chip.(Fig.1) The DNA-based probes target the DNA/RNA sequence of the analyte instead of indirect probing using antibodies. The sensitivity is greatly enhanced by combining the hybridization event with a signal enzyme. The formation of the self-assembled monolayer sensor surface, in-situ DNA hybridization, signal measuring and the sensor regeneration can be performed within 40 minutes. Even without using the PCR, as low as 1000 Escherichia coli (E. coli) cells through 16s rRNA can be detected using this sensor array.(Fig.2) The dimension of each sensor area ranges from 25 mm2 to 160) μm2.