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Application of Chromatography in the Downstream Processing of Biomolecules

2000; Humana Press; Linguagem: Inglês

10.1007/978-1-59259-027-8_7

1940-607X

Mohamed A. Desai, Mark Rayner, Mike Burns, Dervilla Bermingham,

Viral Infectious Diseases and Gene Expression in Insects

The ability to separate and purify biomolecules of interest from a range of complex and diverse biological media such as cell-culture supernatants, fermentation broths, and crude plant and animal extracts has been the cornerstone of biomolecular separation technology. The stringent regulatory requirements for specified purity levels in therapeutic products has been a challenge to the downstream processing practitioners in the biopharmaceutical industry. The increased knowledge, understanding and the development of analytical techniques for the identification of potential biologically hazardous elements and/or components within a biological system, particularly those associated with recombinant technology sources, have rightly imposed an ever more cautious approach to the use of therapeutics in humans.