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First Report of Bacterial Wilt Caused by Ralstonia pseudosolanacearum on Eruca vesicaria subsp. sativa in Brazil
2016; American Phytopathological Society; Volume: 100; Issue: 11 Linguagem: Inglês
10.1094/pdis-04-16-0420-pdn
ISSN1943-7692
AutoresGreecy Mirian Rodrigues Albuquerque, Adriano Márcio Freire Silva, João Roberto Rátis Tenório da Silva, Elineide Barbosa de Souza, Marco Aurélio Siqueira da Gama, R. L. R. Mariano,
Tópico(s)Irrigation Practices and Water Management
ResumoHomePlant DiseaseVol. 100, No. 11First Report of Bacterial Wilt Caused by Ralstonia pseudosolanacearum on Eruca vesicaria subsp. sativa in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Bacterial Wilt Caused by Ralstonia pseudosolanacearum on Eruca vesicaria subsp. sativa in BrazilG. M. R. Albuquerque, A. M. F. Silva, J. R. Silva, E. B. Souza, M. A. S. Gama, and R. L. R. MarianoG. M. R. Albuquerque, A. M. F. Silva, J. R. Silva, E. B. Souza, M. A. S. Gama, and R. L. R. MarianoAffiliationsAuthors and Affiliations G. M. R. Albuquerque A. M. F. Silva J. R. Silva E. B. Souza M. A. S. Gama R. L. R. Mariano , Programa de Pós-graduação em Fitopatologia, Universidade Federal Rural de Pernambuco, 52071-900, Recife, PE, Brazil. Published Online:6 Sep 2016https://doi.org/10.1094/PDIS-04-16-0420-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Arugula (Eruca vesicaria subsp. sativa) is a popular leafy vegetable grown by small farmers in greenbelts of large cities in Brazil. During the rainy season of 2011 (average rainfall 321 mm, average temperature above 25°C), unknown symptoms were observed on leaves of arugula cv. Folha Larga in a 0.25-ha field in Chã Grande County, state of Pernambuco, Brazil. Disease incidence was 5% and infected plants showed dark brown discoloration on leaf veins, small water-soaked lesions near the veins, and reduced size, but did not present the typical green wilt of solanaceous. Bacterial strains CRMRS ruc1, CRMRS ruc2, and CRMRS ruc3 were isolated in NYDA medium and Koch's postulates were completed. For each strain, 10 arugula plants grown in 500-ml plastic pots filled with Basaplant compost were inoculated making a semicircular cut with scalpel on roots inside soil and 15 ml of bacterial suspension (108 CFU/ml) was deposited. Symptoms were observed in 80% of inoculated plants 10 days after inoculation. Control plants treated with water remained asymptomatic. The Biolog Gen III system identified all strains as belonging to the Ralstonia solanacearum complex, and phylotype multiplex PCR (Fegan and Prior 2005) further identified them as R. pseudosolanacearum (Phylotype I). The strains were stored at the Coleção de Culturas Rosa Mariano of the Laboratorio de Fitobacteriologia at the Universidade Federal Rural de Pernambuco. Sequevar of strain CRMRS ruc1 was determined using endoglucanase (egl) partial gene sequences. Cluster analyses by neighbor-joining method between CRMRS ruc1 and reference strains of R. solanacearum complex from GenBank were performed using the algorithm Kimura 2-parameters with 2,000 bootstraps. CRMRS ruc1 showed 100% sequence similarity to strain GMI 1000 of R. pseudosolanacearum sequevar 18. This sequevar was already reported in Pernambuco on other vegetables but not on arugula (Morais et al. 2015). The resulting partial sequence (687 bp) of strain CRMRS ruc1 was deposited in GenBank (KU941947). The studied field was not cultivated with solanaceous for at least 5 years due to the high damages previously caused by bacterial wilt. Thus, we suggest that the pathogen survived in soil and/or in alternative host plants. The importance of this report relies on the register of an additional host for R. pseudosolanacearum, considered one of the top bacteria affecting plants by its diversity and large host range. It also provides information for disease diagnosis in arugula since the symptoms are quite different from the ones seen in bacterial wilt of solanaceous. To our knowledge, this is the first report of R. pseudosolanacearum or any representative of the R. solanacearum species complex as a pathogen of arugula in Brazil and worldwide.References:Fegan, M., and Prior, P. 2005. Page 449 in: Bacterial Wilt Disease and the Ralstonia solanacearum Species Complex. APS Press, St. Paul, Minnesota. Google ScholarMorais, T. M., et al. 2015. Biosci. J. 31:1722. https://doi.org/10.14393/BJ-v31n6a2015-28431 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 11 November 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 7 Oct 2016Published: 6 Sep 2016First Look: 20 Jul 2016Accepted: 6 Jul 2016 Pages: 2319-2319 Information© 2016 The American Phytopathological SocietyCited byBreeding Advances and Prospects in Rocket Salad (Eruca vesicaria ssp. sativa Mill.) Cultivation26 August 2021Sequevar distribution of Ralstonia spp. in Solanaceae in the semiarid climate of the Pernambuco state, Brazil8 November 2020 | European Journal of Plant Pathology, Vol. 159, No. 1Ralstonia solanacearum: Biology and its Management in Solanaceous Vegetable Crops21 January 2021History and Status of Selected Hosts of the Ralstonia solanacearum Species Complex Causing Bacterial Wilt in Brazil13 June 2018 | Frontiers in Microbiology, Vol. 9
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