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In Vitro Multiplication of Ixia flexuosa

1988; American Society for Horticultural Science; Volume: 23; Issue: 6 Linguagem: Inglês

10.21273/hortsci.23.6.1070

2327-9834

H. J. Meyer, J. Van Staden,

Fungal Biology and Applications

Abstract Multiple adventitious buds were induced on corms and corm-derived primary calluses of Ixia flexuosa L. cultured on Murashige and Skoog (MS) medium supplemented with 5 or 10 µ m BA. Buds developed into shoots, nearly half of which rooted in the absence of an auxin. Root formation was increased to 80% with the addition of 2.5 or 5 µ m NAA to the MS medium. Almost two-thirds of the plantlets survived hardening in a mistbed. Thidiazuron at 0.1 or 1.0 mg·liter −1 also induced adventitious buds on corm explants, but these buds were less vigorous than those produced with BA. Chemical names used: N -phenylmethyI)-1 H -purin-6-amine (BA); 1-naphthaleneacetic acid (NAA); N -phenyl- N' -1,2,3-thiadiazol-5-ylurea (thidiazuron).