Separation of Different Kinds of Nucleated Cells from Blood by Centrifugal Elutriation
1982; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-564501-0.50012-2
Autores Tópico(s)Field-Flow Fractionation Techniques
ResumoPublisher Summary Centrifugal elutriation (CE), or counterflow centrifugation, has been used to isolate pure, or at least highly concentrated, populations of various blood leukocytes. The technique involves the balancing of outwardly directed inertial forces acting on cells in a centrifugal field against inwardly directed hydrodynamic forces created by the continuous pumping of a fluid through a chamber in the centrifuge rotor in an inward radial direction. The physical properties of a cell which are most significant in determining its sedimentation rate in a counterflow centrifuge are its volume and density or, more precisely, buoyant density, which may be defined as the difference between the specific gravity of the cell and that of the eluting medium. Mononuclear cells can be separated from polymorhonuclear cells purely on the basis of density. Granulocytes are the most numerous leukocytes and have at the same time generally higher sedimentation rates than mononuclear cells.
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