Artigo Acesso aberto Revisado por pares

A proposal for validation of antibodies

2016; Nature Portfolio; Volume: 13; Issue: 10 Linguagem: Inglês

10.1038/nmeth.3995

ISSN

1548-7105

Autores

Mathias Uhlén, Anita Bandrowski, Steven A. Carr, A.M. Edwards, Jan Ellenberg, Emma Lundberg, David L. Rimm, Henry Rodriguez, Tara Hiltke, M Snyder, Tadashi Yamamoto,

Tópico(s)

Advanced Biosensing Techniques and Applications

Resumo

We convened an ad hoc International Working Group for Antibody Validation in order to formulate the best approaches for validating antibodies used in common research applications and to provide guidelines that ensure antibody reproducibility. We recommend five conceptual 'pillars' for antibody validation to be used in an application-specific manner.

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