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Effects of nickel on the fatty acid composition of the diatom Phaeodactylum tricornutum

2016; Frontiers Media; Volume: 3; Linguagem: Inglês

10.3389/conf.fmars.2016.04.00033

2296-7745

Matos Ana Rita, Gameiro Carla, Duarte Bernardo, Caçador Isabel, Maria Teresa Cabrita,

Diatoms and Algae Research

Event Abstract Back to Event Effects of nickel on the fatty acid composition of the diatom Phaeodactylum tricornutum Ana Rita Matos1*, Carla L. Gameiro2, Bernardo Duarte2, Isabel Caçador2 and Maria Teresa Cabrita3, 4 1 BioISI - Instituto de Biosistemas & Ciências Integrativas (BioISI), Departamento de Biologia Vegetal da Faculdade de Ciências da Universidade de Lisboa (FCUL), Portugal 2 MARE - Centro de Ciências do Mar e Ambiente, Faculdade de Ciências da Universidade de Lisboa (FCUL), Portugal 3 IPMA - Instituto Português do Mar e da Atmosfera, Portugal 4 Centro de Estudos Geográficos, Instituto de Geografia e Ordenamento do Território, Universidade de Lisboa, Portugal Phytoplankton produces a wide range of bioactive molecules that play physiological roles allowing cells to deal with changes in environmental conditions. In particular, the lipid composition of cell membranes and fatty acid unsaturation vary accordingly with environmental factors. Because essential fatty acids (EFA) produced exclusively by the plant kingdom support the production of polyunsaturated fatty acids (PUFA) at higher trophic levels of marine food webs, changes in fatty acid composition of phytoplankton can cause alterations in the structure of food chains, with possible impacts on the fish stocks of commercial interest. In the present work we have analyzed the fatty acid composition, by gas chromatography, of the marine diatom Phaeodactylum tricornutum, grown under standard control conditions or exposed to nickel (Ni). The major fatty acids present in P. tricornutum are the saturated myristic (C14:0) and palmitic (C16:0) acids, the monounsaturated palmitoleic acid (C16:1), the di-unsaturated C16:2, the tri-unsaturated C16:3 and the long chain (LC) PUFA eicosapentaenoic acid (EPA, C20:5). Our results show that Ni had a significant negative impact in the relative proportion of polyunsaturated fatty acids C16:2, C16:3 and EPA. Moreover, optimization of a straightforward thin layer chromatography procedure, coupled to GC analysis, developed for this model diatom, revealed an altered balance between membrane and storage lipids classes, with particular impacts of Ni on plastidial lipids. To evaluate the effect of Ni induced oxidative stress, on membrane lipids, detection of lipid hydroperoxydes was performed by the FOX method, which confirmed that these compounds accumulated to a further extent in Ni-exposed cells. In conclusion, we show that Ni causes a decrease in the PUFA content of cultured P. tricornutum cells, due, at least in part, to Ni-induced oxidative stress. Extending these studies to samples grown under natural conditions will contribute to evaluate the impact of metal contamination on the physiology and nutritional value of marine food webs. Acknowledgements This work was financed by the Marine and Environmental Sciences Centre (MARE) (project UID/MAR/04292/2013) and the Biosystems and Integrative Sciences Institute (BioISI) (project UID/MULTI/04046/2013), and grants to B. Duarte (SFRH/BD/75951/2011), and M.T. Cabrita (SFRH/BPD/50348/2009), all from Fundação para a Ciência e Tecnologia (FCT). Keywords: phaeodactylum tricornutum, Nickel, Oxidative Stress, Fatty Acids, Lipids Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016. Presentation Type: Poster presentation Topic: Aquaculture Citation: Matos A, Gameiro CL, Duarte B, Caçador I and Cabrita M (2016). Effects of nickel on the fatty acid composition of the diatom Phaeodactylum tricornutum. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00033 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2016; Published Online: 13 Jul 2016. * Correspondence: PhD. Ana Rita Matos, BioISI - Instituto de Biosistemas & Ciências Integrativas (BioISI), Departamento de Biologia Vegetal da Faculdade de Ciências da Universidade de Lisboa (FCUL), Lisboa, 1749-016, Portugal, armatos@fc.ul.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ana Rita Matos Carla L Gameiro Bernardo Duarte Isabel Caçador Maria Teresa Cabrita Google Ana Rita Matos Carla L Gameiro Bernardo Duarte Isabel Caçador Maria Teresa Cabrita Google Scholar Ana Rita Matos Carla L Gameiro Bernardo Duarte Isabel Caçador Maria Teresa Cabrita PubMed Ana Rita Matos Carla L Gameiro Bernardo Duarte Isabel Caçador Maria Teresa Cabrita Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.