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Studies on filariasis VII : Histological observation on the encapsulated Brugia malayi larvae in the abdominal haemocoel of the mosquitoes, Armigeres subalbatus

1986; Japan Society of Medical Entomology and Zoology; Volume: 37; Issue: 1 Linguagem: Inglês

10.7601/mez.37.59

2185-5609

Mutsuo Kobayashi, Nobuo OGURA, Hitomi Tsuruoka, Yuichi Chigusa, Shogi Mishima,

Insects and Parasite Interactions

Histological observations of Brugia malayi infected mosquitoes, Armigeres subalbatus, prepared at 0.5,3,12,24,48hrs and on 3,5,7,10 days after blood meal, were carried out by routine paraffin sectioning and hematoxylineosin staining method. Light brown substances around microfilari-form larvae were observed as early as 30min post-infection and some parts of them had begun to change in color from light brown to dark brown. Until 90min post-infection almost all of the larvae in the abdominal haemocoel were enclosed in a dark brown capsule. Thereafter, many round shaped cells were observed attached on the pigmented capsule until about 3hrs post-infection. The adhered cells became distinctly flattened by 24hrs post-infection and the nucleus of the individual cell became difficult to be recognized. After 48hrs post-infection, cell layer around the pigmented capsule was recognized as a faintly basophilic or brownish layer. Inside the cell layer many minute brown particles were observed from 5 to 10 day post-infection. It was clarified that cellular response by host mosquitoes lagged behind the pigmentation and that the cells of mosquitoes played a role in a series of encapsulation reactions against filarial larvae in the abdominal haemocoel.