First Report of Colletotrichum siamense Causing Anthracnose of Cliff Banana in India
2016; American Phytopathological Society; Volume: 101; Issue: 2 Linguagem: Inglês
10.1094/pdis-07-16-0961-pdn
ISSN1943-7692
AutoresV. Kumar, B. A. Nair, P. V. R. Nair, Annamalai Arunachalam, R. Jaishanker, K. Umamaheswaran, N. P. Sooraj, C. K. Peethambaran,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 101, No. 2First Report of Colletotrichum siamense Causing Anthracnose of Cliff Banana in India PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum siamense Causing Anthracnose of Cliff Banana in IndiaV. S. Kumar, B. A. Nair, P. V. R. Nair, A. Annamalai, R. Jaishanker, K. Umamaheswaran, N. P. Sooraj, and C. K. PeethambaranV. S. Kumar, B. A. Nair, P. V. R. Nair, A. Annamalai, R. Jaishanker, K. Umamaheswaran, N. P. Sooraj, and C. K. PeethambaranAffiliationsAuthors and Affiliations V. S. Kumar , Department of Biotechnology, School of Biotechnology and Health Science, Karunya University, Coimbatore 641114, India B. A. Nair , Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram, India P. V. R. Nair , Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia A. Annamalai , Department of Biotechnology, School of Biotechnology and Health Science, Karunya University, Coimbatore 641114, India R. Jaishanker , School of Ecological Informatics, Indian Institute of Information Technology and Management, Kerala, Thiruvananthapuram 695581, India K. Umamaheswaran , Department of Plant Pathology, College of Agriculture, Vellayani, Thiruvananthapuram, India N. P. Sooraj , School of Ecological Informatics, Indian Institute of Information Technology and Management, Kerala, Thiruvananthapuram 695581, India C. K. Peethambaran , School of Ecological Informatics, Indian Institute of Information Technology and Management, Kerala, Thiruvananthapuram 695581, India. Published Online:13 Dec 2016https://doi.org/10.1094/PDIS-07-16-0961-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Cliff banana, Ensete superbum (Roxb.) Cheesman of the family Musaceae, is a non-stoloniferous herb that reproduces only through seeds. It is a rare and threatened plant species in India used for medicine, food, fiber, and ornamental purposes (Sarojkumar et al. 2015). Cliff banana is distributed on rocky slopes and crevices across the forests of the Western Ghats and northeastern sub-Himalayan tracts of India (Cheesman 1947). In July 2011, 1-month-old cliff banana plants with black sunken lesions on midribs and petioles of young and old leaves were observed in Vithura (8°40′51.6″N, 77°06′07.2″E) and Kottiyur (11°50′20.4″N, 75°55′01.2″E) forest regions located in Kerala State, India. The disease was subsequently found scattered across an area of 2 km2. Symptoms initially appeared as water soaked, dark brown or black, sunken, circular to irregular lesions usually 25 mm or more in diameter extending from the midrib to across the leaf lamina. Infection on the leaf sheath and petiole resulted in the weakening and subsequent breaking off at the point of infection. At high humidity, infection expanded rapidly; plants turned yellow and gradually dried up. Infected leaves and midribs were surface sterilized in 0.5% NaClO for 1 min and rinsed in sterile distilled water. Tissues were plated on potato dextrose agar (PDA) and incubated under alternating light and dark at 27°C for 3 days. For identification, single-spore isolates were grown on PDA at 28°C. Initially the colonies were white and after 6 days became brownish to pinkish with the entire margin showing zonations. Conidia were formed in masses, hyaline, and fusiform with obtuse ends. Average conidial size was 15 × 4 μm (range of 9.5 to 20 μm long and 3 to 5 μm wide) and morphology was consistent with the description of C. siamense (Prihastuti et al. 2009). For molecular characterization, sequence of the ApMat region was obtained at Institute of Microbial Technology (MTCC), Chandigarh, India. The isolate was confirmed as C. siamense and deposited in GenBank (KX186996). BLASTn analysis of ApMat sequence showed 98% of identity with the type specimen of C. siamense (KP703769) (Liu et al. 2016). C. siamense culture was deposited at the Indian Type Culture Collection Identification, Indian Agriculture Research Institute, New Delhi (accession no. C9-8605.11/11) and MTCC (accession no. 38196). Pathogenicity tests were conducted on 1-month-old cliff banana seedlings. Eight seedlings were planted in 2.8-liter pots; 12 plants were sprayed with a spore suspension of 106 spores/ml of C9-8606 isolate. Seedlings were sprayed with sterile water to serve as controls, then placed on poly house benches at the Department of Plant Pathology, College of Agriculture, Thiruvananthapuram, India. Anthracnose symptoms on leaves were observed at 7 days after inoculation, which was similar to the original disease. The fungus was reisolated from the necrotic lesions, confirming Koch's postulates. Seedlings from the control treatment remained symptomless. The fungus C. siamense infects many tropical grown fruits and flower species (Liu et al. 2016). To our knowledge, this is the first report of C. siamense causing anthracnose on cliff banana.References:Cheesman, E. E. 1947. Kew Bull. 2:97. https://doi.org/10.2307/4109206 Crossref, Google ScholarLiu, F., et al. 2016. BMC Evol. Biol. 16:81. https://doi.org/10.1186/s12862-016-0649-5 Crossref, ISI, Google ScholarPrihastuti, H., et al. 2009. Fungal Divers. 39:89. ISI, Google ScholarSarojkumar, V., et al. 2015. J. Ayur. Herb. Medic. 1:54. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 2 February 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 13 Jan 2017Published: 13 Dec 2016First Look: 23 Aug 2016Accepted: 15 Aug 2016 Pages: 390-390 Information© 2017 The American Phytopathological SocietyCited byIdentification and Observation of Infection Processes of Colletotrichum Species Associated with Pearl Plum Anthracnose in Guangxi, ChinaRong Huang, Qing Gui, Yujie Zhang, Wenxiu Sun, Lihua Tang, Suiping Huang, Tangxun Guo, Qili Li, Jianyou Mo, Huiye Huang, Mingzhong Fan, Zongbin Zhang, and Tom Hsiang20 November 2022 | Plant Disease, Vol. 106, No. 12Pest categorisation of Colletotrichum aenigma, C. alienum, C. perseae, C. siamense and C. theobromicolaEFSA Journal, Vol. 20, No. 8Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1First Record of Colletotrichum gloeosporioides Causing Anthracnose of Banana in PakistanM. 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