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VTA dopaminergic neurons regulate ethologically relevant sleep–wake behaviors

2016; Nature Portfolio; Volume: 19; Issue: 10 Linguagem: Inglês

10.1038/nn.4377

1546-1726

Ada Eban-Rothschild, Gideon Rothschild, William J. Giardino, Jeff R. Jones, Luı́s de Lecea,

Neural dynamics and brain function

Motivated behaviors are critically dependent upon arousal but little is known about the neuronal mechanisms that coordinate motivational processes with sleep–wake regulation. The authors demonstrate that VTA dopaminergic neurons, which are central regulators of motivational processes, bidirectionally regulate sleep–wake states and sleep-related nesting behavior. Dopaminergic ventral tegmental area (VTA) neurons are critically involved in a variety of behaviors that rely on heightened arousal, but whether they directly and causally control the generation and maintenance of wakefulness is unknown. We recorded calcium activity using fiber photometry in freely behaving mice and found arousal-state-dependent alterations in VTA dopaminergic neurons. We used chemogenetic and optogenetic manipulations together with polysomnographic recordings to demonstrate that VTA dopaminergic neurons are necessary for arousal and that their inhibition suppresses wakefulness, even in the face of ethologically relevant salient stimuli. Nevertheless, before inducing sleep, inhibition of VTA dopaminergic neurons promoted goal-directed and sleep-related nesting behavior. Optogenetic stimulation, in contrast, initiated and maintained wakefulness and suppressed sleep and sleep-related nesting behavior. We further found that different projections of VTA dopaminergic neurons differentially modulate arousal. Collectively, our findings uncover a fundamental role for VTA dopaminergic circuitry in the maintenance of the awake state and ethologically relevant sleep-related behaviors.