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Ibrutinib modifies the function of monocyte/macrophage population in chronic lymphocytic leukemia

2016; Impact Journals LLC; Volume: 7; Issue: 40 Linguagem: Inglês

10.18632/oncotarget.11782

1949-2553

Stefania Fiorcari, Rossana Maffei, Valentina Audrito, Silvia Martinelli, Elisa ten Hacken, Patrizia Zucchini, Giulia Grisendi, Leonardo Potenza, Mario Luppi, Jan A. Burger, Silvia Deaglio, Roberto Marasca,

Chronic Myeloid Leukemia Treatments

// Stefania Fiorcari 1, * , Rossana Maffei 1, * , Valentina Audrito 2 , Silvia Martinelli 1 , Elisa ten Hacken 3 , Patrizia Zucchini 1 , Giulia Grisendi 1 , Leonardo Potenza 1 , Mario Luppi 1 , Jan A. Burger 3 , Silvia Deaglio 2 , Roberto Marasca 1 1 Hematology Unit, Department of Medical and Surgical Sciences, University of Modena and Reggio Emilia, Modena, Italy 2 Department of Medical Sciences, University of Turin and Human Genetics Foundation, Turin, Italy 3 Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, USA * These authors have contributed equally to this work Correspondence to: Roberto Marasca, email: roberto.marasca@unimore.it Stefania Fiorcari, email: stefania.fiorcari@unimore.it Keywords: CLL, ibrutinib, microenvironment, nurse-like cells, immune modulation Received: June 08, 2016 Accepted: August 15, 2016 Published: September 01, 2016 ABSTRACT In lymphoid organs, nurse-like cells (NLCs) show properties of tumor-associated macrophages, playing a crucial role in chronic lymphocytic leukemia (CLL) cell survival. Ibrutinib, a potent inhibitor of Bruton's tyrosine kinase (BTK), is able to counteract pro-survival signals in CLL cells. Since the effects on CLL cells have been studied in the last years, less is known about the influence of ibrutinib on NLCs properties. We sought to determine how ibrutinib modifies NLCs functions focusing on the balance between immunosuppressive and inflammatory features. Our data show that ibrutinib targets BTK expressed by NLCs modifying their phenotype and function. Treatment with ibrutinib reduces the phagocytic ability and increases the immunosuppressive profile of NLCs exacerbating the expression of M2 markers. Accordingly, ibrutinib hampers LPS-mediated signaling, decreasing STAT1 phosphorylation, while allows IL-4-mediated STAT6 phosphorylation. In addition, NLCs treated with ibrutinib are able to protect CLL cells from drug-induced apoptosis partially through the secretion of IL-10. Results from patient samples obtained prior and after 1 month of treatment with ibrutinib show an accentuation of CD206, CD11b and Tie2 in the monocytic population in the peripheral blood. Our study provides new insights into the immunomodulatory action of ibrutinib on monocyte/macrophage population in CLL.