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The marine invasive Asparagopsis armata (Harvey, 1855) as source of bioactive valuable compounds - Antioxidant potential enrichment by Vacuum liquid Chromatography

2016; Frontiers Media; Volume: 3; Linguagem: Inglês

10.3389/conf.fmars.2016.04.00067

2296-7745

Susete Pintéus, Rodrigues Agnieszka, Joana Silva, Lokman Christien, Lemos Marco, Rui Pedrosa,

Aquaculture Nutrition and Growth

Event Abstract Back to Event The marine invasive Asparagopsis armata (Harvey, 1855) as source of bioactive valuable compounds - Antioxidant potential enrichment by Vacuum liquid Chromatography Susete Pinteus1*, Agnieszka N. Rodrigues2, Joana Silva1, Christien Lokman2, Marco F. Lemos1 and Rui Pedrosa1 1 MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Portugal 2 HAN University of Applied Sciences, Netherlands Several seaweeds have the ability to develop unique metabolic strategies to ensure survival in new environments and further spreading. Among others, these strategies encompass the production of secondary metabolites which have shown potent bioactivities. Asparagopsis armata (Harvey, 1855), a Rhodophyta that belongs to Bonnemaisoniaceae family, is a marine invasive seaweed, native from Australian coast that has spread along the Portuguese coast. This seaweed is known to be a rich source of halogenated compounds with different biological activities, including antioxidant activity [1-3]. This study evaluates the antioxidant properties of a methanol:dichloromethane (1:1) extract of Asparagopsis armata collected in the Peniche coast, and the potential of vacuum liquid chromatography (VLC) on silica gel for further concentration of the antioxidant compounds. VLC was performed using cyclohexane with increasing amounts of ethyl acetate (F1 – F5) and three fractions with dichloromethane, methanol and 1:1 of both (F6 - F8). The antioxidant activity of all fractions was accessed by testing the DPPH free radical scavenging activity, the oxygen radical absorbance capacity (ORAC), the reducing power (RP), and also through the quantification of the total phenolic content (TPC). A Principal Components Analysis (PCA) was carried out to obtain an overview of the results obtained in all the tests and to investigate the relationships among TPC and the different antioxidant activity assays. The results were the most expressive in the ORAC method, with all the fractions presenting high radical absorbance capacity with values ranging from ≈1000 to 2300 µmol Trolox equivalents per gram of extract, suggesting a strong potential for scavenging peroxyl radicals. All altogether, the results obtained in the antioxidant activity assays presented a strong correlation, and the fractions F5>F4>F3 exhibited the strongest antioxidant properties, revealing to be the most relevant fur further purification and characterization. Our results suggest that vacuum liquid chromatography is a useful technique for the concentration of antioxidant compounds, revealing the potential of A. armata as a source for novel antioxidant molecules. Acknowledgements Susete Pinteus and Joana Silva are financial supported by a grant from FCT - Fundação para a Ciência e Tecnologia (SFRH/BD/96203/2013, SFRH/BD/103255/2014, respectively). References 1. McConnell, O., Fenical, W. (1977a). Halogen chemistry of the red alga Asparagopsis. Phytochemistry 16, 367-374. 2. McConnell, O., Fenical, W. (1980). Halogen chemistry of the red alga Bonnemaisonia. Phytochemistry. 19, 233-247. 3. Rhimou, Bouhlal, Riadi Hassane, and Bourgougnon Nathalie. "Antioxidant activity of Rhodophyceae extracts from Atlantic and Mediterranean Coasts of Morocco." African Journal of Plant Science 7.3 (2013): 110-117. Keywords: seaweeds, Phenolic content, Marine invasive species, Marine Natural Products, Marine bioactive compounds Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016. Presentation Type: Poster presentation Topic: Blue Biotechnology Citation: Pinteus S, Rodrigues AN, Silva J, Lokman C, Lemos MF and Pedrosa R (2016). The marine invasive Asparagopsis armata (Harvey, 1855) as source of bioactive valuable compounds - Antioxidant potential enrichment by Vacuum liquid Chromatography. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00067 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2016; Published Online: 13 Jul 2016. * Correspondence: Dr. Susete Pinteus, MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Peniche, 2520–641, Portugal, susete.pinteus@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Susete Pinteus Agnieszka N Rodrigues Joana Silva Christien Lokman Marco F Lemos Rui Pedrosa Google Susete Pinteus Agnieszka N Rodrigues Joana Silva Christien Lokman Marco F Lemos Rui Pedrosa Google Scholar Susete Pinteus Agnieszka N Rodrigues Joana Silva Christien Lokman Marco F Lemos Rui Pedrosa PubMed Susete Pinteus Agnieszka N Rodrigues Joana Silva Christien Lokman Marco F Lemos Rui Pedrosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.