Portal de Periódicos da CAPES

Study of the Anti-cancer Effects of Hyperthermia with Immunohistochemical Technique using Anti-BrdU Monoclonal Antibody.

1991; Volume: 7; Issue: 2 Linguagem: Inglês

10.3191/thermalmedicine.7.131

1881-9516

Makoto Nakanishi, Motohiko Fukushima, Kazushige Arai, Tadashi Koike,

Monoclonal and Polyclonal Antibodies Research

The anti-cancer effects of hyperthermia were studied at various post-treatment intervals after heating with Immunohistochemical technique in vivo and in vitro using Anti-Bromodeoxyridine (BrdU) monoclonal antibody which is said to identify S-phase cells and evaluate their growth potential.VX2 carcinoma cells at 1.5 × 107 were trasplanted into the both legs of each rabbit and the heating were carried out for 40 minutes in a homothermic water bath, keeping the temperature of the tumors at higher than 43°C.In vivo and in vitro, we obtained similar results as follows. In the BrdU stain of control group we found many positive S-phase cells uniformly in wide area.BrdU stain of the group treated soon after heating showed no positive cell in the tumors. BrdU stain of the group treated 12 hours after heating showed some positive cells only in tumor periphery, and the group treated 48 hours after heating came to show a few positive cells in the tumor center, too.BrdU stain of the group treated 5 days after heating showed many positive cells, nearly as the control group.By this study using BrdU stain we may say, from the viewpoint of cell dynamics, that S-phase tmour cells are injured immediately by heating and regrow from peripheral parts with the lapse of time.Immunohistochemical detection of S-phase cells using BrdU stain may thus be useful for evaluating the heating sensitivity of cancer.