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Detecting multiple DNA human profile from a mosquito blood meal

2016; Research Foundation of Ribeirão Preto; Volume: 15; Issue: 3 Linguagem: Inglês

10.4238/gmr.15037547

1676-5680

K.C.N. Rabêlo, Cleide Maria Ribeiro de Albuquerque, V.B. Tavares, S.M. Santos, C.A. Souza, T.C. Oliveira, Ronald Moura, Lucas André Cavalcanti Brandão, Sérgio Crovella,

Molecular Biology Techniques and Applications

Criminal traces commonly found at crime scenes may present mixtures from two or more individuals.The scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime.Thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid in suspect investigation.The aim of Genetics and Molecular Research 15 (3): gmr.15037547 the study was to obtain a single Aedes aegypti mosquito profile from a human DNA mixture containing genetic materials of four individuals.We also determined the effect of blood acquisition time by setting time intervals of 24, 48, and 72 h after the blood meal.STR loci and amelogenin were analyzed, and the results showed that human DNA profiles could be obtained from hematophagous mosquitos at 24 h following the blood meal.It is possible that hematophagous mosquitoes can be used as biological remains at the scene of the crime, and can be used to detect human DNA profiles of up to four individuals.