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Biochemical and biophysical tools for the detection of trace metal stress in the diatom model species Phaeodactylum tricornutum

2016; Frontiers Media; Volume: 3; Linguagem: Inglês

10.3389/conf.fmars.2016.04.00090

2296-7745

Maria Teresa Cabrita, Duarte Bernardo, Carla Gameiro, Utkin Andrei, Matos Ana Rita, Caçador Isabel, Paulo Cartaxana,

Electrochemical sensors and biosensors

Event Abstract Back to Event Biochemical and biophysical tools for the detection of trace metal stress in the diatom model species Phaeodactylum tricornutum Maria Teresa Cabrita1, 2*, Bernardo Duarte3, Carla L. Gameiro3, Andrei B. Utkin4, 5, Ana Rita Matos6, Isabel Caçador3 and Paulo Cartaxana7 1 IPMA - Instituto Português do Mar e da Atmosfera, Portugal 2 Centro de Estudos Geográficos, Instituto de Geografia e Ordenamento do Território, Universidade de Lisboa, Portugal 3 MARE - Centro de Ciências do Mar e do Ambiente, Faculdade de Ciências da Universidade de Lisboa (FCUL), Portugal 4 INOV-INESC Inovação, Portugal 5 ICEMS, IST - Universidade Técnica de Lisboa, Portugal 6 BioISI - Instituto de Biosistemas& Ciências Integrativas, Departamento de Biologia Vegetal da Faculdade de Ciências da Universidade de Lisboa, Portugal 7 University of Copenhagen, Department of Biology Marine Biological Section, Denmark This work reports changes in cell number, growth rate, trace metal content, laser-induced fluorescence (LIF) spectra, PS II overall activity and efficiency during light harvesting, electron transport chain (ETC) behaviour, OJIP-transient light curves, and pigment profiles of Phaeodactylum tricornutum exposed to Co, Ni, Cu, Cd, Hg, Pb, and to a mixture of all elements combined (Mix). Decreases in LIF intensity in the far-red region, large deviations in wavelength emission maxima in the red region of LIF spectra, and significant increase in the F685/F735 ratio, indicated alterations in the photosystem II efficiency, impairment in the distribution of excitation energy between the two photosystems, Chl a damage and potential structural changes, for all metals and more accentuated for Hg in exposed cells. This study clearly shows F685/F735 ratio and the deviations in wavelength emission maxima as adequate metal stress indicators. LIF-based techniques can be used as time-saving, highly sensitive and effective alternative tool for the detection of trace metal stress, with potential for remote sensing and metal contamination screening in marine coastal areas. In alignment with this, OJIP-test parameter analysis corroborated these results, showing that trace metals generated differential photobiological responses at different levels of the energy transduction pathway. In fact, this fluorometry technique proved to be an efficient tool with potential to discriminate the effects of different metals. These physiological changes point out P. tricornutum as potential sentinel species for metal contamination and show that the set of parameters used to assess them can be employed as useful and efficient battery of biomarkers of metal stress for the early detection of metal contamination in marine systems. Acknowledgements This work was financed by the Marine and Environmental Sciences Centre (MARE) (project UID/MAR/04292/2013) and the Biosystems and Integrative Sciences Institute (BioISI) (project UID/MULTI/04046/2013), and grants to B. Duarte (SFRH/BD/75951/2011), and M.T. Cabrita (SFRH/BPD/50348/2009), all from Fundação para a Ciência e Tecnologia (FCT). Keywords: Biochemical and biophysical tools, phaeodactylum tricornutum, metal stress detection, biomarkers, Marine coastal areas Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016. Presentation Type: Poster presentation Topic: Biodiversity, Conservation and Coastal Management Citation: Cabrita M, Duarte B, Gameiro CL, Utkin AB, Matos A, Caçador I and Cartaxana P (2016). Biochemical and biophysical tools for the detection of trace metal stress in the diatom model species Phaeodactylum tricornutum. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00090 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 May 2016; Published Online: 13 Jul 2016. * Correspondence: PhD. Maria Teresa Cabrita, IPMA - Instituto Português do Mar e da Atmosfera, Lisboa, 1449-006, Portugal, tcabrita@campus.ul.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Maria Teresa Cabrita Bernardo Duarte Carla L Gameiro Andrei B Utkin Ana Rita Matos Isabel Caçador Paulo Cartaxana Google Maria Teresa Cabrita Bernardo Duarte Carla L Gameiro Andrei B Utkin Ana Rita Matos Isabel Caçador Paulo Cartaxana Google Scholar Maria Teresa Cabrita Bernardo Duarte Carla L Gameiro Andrei B Utkin Ana Rita Matos Isabel Caçador Paulo Cartaxana PubMed Maria Teresa Cabrita Bernardo Duarte Carla L Gameiro Andrei B Utkin Ana Rita Matos Isabel Caçador Paulo Cartaxana Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.