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An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts

2016; Elsevier BV; Volume: 17; Issue: 2 Linguagem: Inglês

10.1016/j.scr.2016.09.015

1876-7753

Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon D. Hennebold, Kyle E. Orwig, Amander T. Clark,

Virus-based gene therapy research

We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.