Statistical modeling and optimization of cellulase production by Bacillus licheniformis NCIM 5556 isolated from the hot spring, Maharashtra, India
2016; Elsevier BV; Volume: 29; Issue: 3 Linguagem: Inglês
10.1016/j.jksus.2016.08.001
ISSN2213-686X
AutoresS. Shanavas, I. Ganesh Moorthy, Nallusamy Sivakumar, Gopal Selvakumar,
Tópico(s)Enzyme Production and Characterization
ResumoCellulase shows great interest in the field of organic acids, and biotechnology industries. Cellulase-producing thermophilic bacteria was isolated from West Coast hot spring (Rajapur, Ratnagiri District of Maharashtra (Lat. 16°38′42″N; long. 73°31′53″E) and identified as Bacillus licheniformis. Out of seven variables in the cellulase production medium, four variables such as carboxy methyl cellulose (CMC), calcium chloride, Tween-20, and temperature were screened through Plackett–Burman design and further optimized through response surface methodology (RSM) for higher cellulase production. The optimal conditions were found to be CMC, 19.21 g/L, CaCl2·6H2O, 25.06 mg/L, Tween-20, 2.96 mL/L and temperature 43.35 °C. The high cellulase production 42.99 IU/mL was achieved in a 7 L scale bio fermenter using optimal conditions. A 3 fold increase in cellulase production was achieved by RSM model. These methods have proven suitable to optimize cellulase production by a thermophilic Bacillus.
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