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Mitochondrial uncoupler carbonyl cyanide m‐chlorophenylhydrazone induces vasorelaxation without involving K ATP channel activation in smooth muscle cells of arteries

2016; Wiley; Volume: 173; Issue: 21 Linguagem: Inglês

10.1111/bph.13578

1476-5381

Yan‐Qiu Zhang, Xin Shen, Xiao‐Lin Xiao, Mingyu Liu, Shan‐Liang Li, Jie Yan, Jing Jin, Jin‐Lai Gao, Changlin Zhen, Nan Hu, Xinzi Zhang, Yu Tai, Liang‐Shuan Zhang, Yunlong Bai, De‐Li Dong,

Adipose Tissue and Metabolism

Background and Purpose The effects and mechanisms of chemical mitochondrial uncouplers on vascular function have never been identified. Here, we characterized the effects of the typical mitochondrial uncoupler carbonyl cyanide m‐chlorophenylhydrazone (CCCP) on vascular function in rat mesenteric arteries and aorta and elucidated the potential mechanisms. Experimental Approach Isometric tension of mesenteric artery and thoracic aorta was recorded by using a multiwire myograph system. Protein levels were measured by western blot analyses. Cytosolic [Ca 2 + ] i , mitochondrial ROS (mitoROS) and mitochondrial membrane potential of smooth muscle cells (A10) were measured by laser scanning confocal microscopy. Key Results Acute treatment with CCCP relaxed phenylephrine (PE)‐ and high K + (KPSS)‐induced constriction of rat mesenteric arteries with intact and denuded endothelium. Pretreatment with CCCP prevented PE‐ and KPSS‐induced constriction of rat mesenteric arteries with intact and denuded endothelium. Similarly, CCCP prevented PE‐ and KPSS‐induced constriction of rat thoracic aorta. CCCP increased the cellular ADP/ATP ratio in vascular smooth muscle cells (A10) and activated AMPK in A10 cells and rat thoracic aorta tissues. CCCP‐induced aorta relaxation was attenuated in AMPK α1 knockout (−/−) mice. SERCA inhibitors thapsigargin and cyclopiazonic acid (CPA) but not the K ATP channel blocker glibenclamide partially inhibited CCCP‐induced vasorelaxation in endothelium‐denuded rat mesenteric arteries. CCCP increased cytosolic [Ca 2 + ] i , mitoROS production and depolarized mitochondrial membrane potential in A10 cells. FCCP, the analogue of CCCP, had similar vasoactivity as CCCP in rat mesenteric arteries. Conclusions and Implications CCCP induces vasorelaxation by a mechanism that does not involve K ATP channel activation in smooth muscle cells of arteries.