Effects of Thidiazuron on Grapevine Axillary Buds Cultivated in Vitro
1991; American Society for Horticultural Science; Volume: 26; Issue: 8 Linguagem: Inglês
10.21273/hortsci.26.8.1083
ISSN2327-9834
Autores Tópico(s)Plant Pathogens and Fungal Diseases
ResumoTable 1. Effects of TDZ on proliferation and rooting of grapevine nodal segments after 1 month of culture in vitro. Morphological visual evaluation rating: from 0 (strongly vitrified and/or malformed shoots) to 4 (fully normal and healthy shoots). N -phenylN’ -1,2,3-thiadiazol-5-ylurea [thidiazuron (TDZ)], a newly available phenylurea, has been reported to have high cytokinin activity at low concentrations. Its cytokinin-like effects on several species include promotion of growth in cell and callus cultures, stimulation of in vitro shoot proliferation and somatic embryogenesis, and budbreak of dormant trees (Matsuda and Hirabayashi, 1989; Mok et al., 1987). The aim of this work is to test grapevine response to TDZ during in vitro culture of axillary buds. Woody cuttings of Vitis vinifera L. ‘Barbera’ (clone CVT-AT84) were forced to sprout. After 6 weeks, the new shoots were collected and sterilized by immersion in a 25% (v/v) aqueous solution of a 7% chlorine detergent for 15 min. Explants, isolated from the sterilized shoots, were nodal segments formed by the axillary buds plus a small part of the adjacent tissues of stem and petiole (0.8 cm average length). Buds were cultured on an agarized Nitsch and Nitsch (1969) medium, modified by increasing thiamine HCl to 15 μM and FeEDTA to 200 μM, and containing 10, 1, 0.1, 0.01, 0.001, or 0 μ M TDZ (Schering S.p.A., Segrate, Italy). The pH of the media was adjusted to 5.6 before autoclaving. Cultures were grown at 23C with a 16-h photoperiod and 50 μmol·s -1· m-2
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