Electronic cigarette aerosol induces significantly less cytotoxicity than tobacco smoke
2016; Taylor & Francis; Volume: 26; Issue: 6 Linguagem: Inglês
10.1080/15376516.2016.1217112
ISSN1537-6524
AutoresDavid Azzopardi, Kharishma S. Patel, Tomasz Jaunky, Simone Santopietro, Oscar M. Camacho, John McAughey, Marianna Gaça,
Tópico(s)Air Quality and Health Impacts
ResumoElectronic cigarettes (E-cigarettes) are a potential means of addressing the harm to public health caused by tobacco smoking by offering smokers a less harmful means of receiving nicotine. As e-cigarettes are a relatively new phenomenon, there are limited scientific data on the longer-term health effects of their use. This study describes a robust in vitro method for assessing the cytotoxic response of e-cigarette aerosols that can be effectively compared with conventional cigarette smoke. This was measured using the regulatory accepted Neutral Red Uptake assay modified for air-liquid interface (ALI) exposures. An exposure system, comprising a smoking machine, traditionally used for in vitro tobacco smoke exposure assessments, was adapted for use with e-cigarettes to expose human lung epithelial cells at the ALI. Dosimetric analysis methods using real-time quartz crystal microbalances for mass, and post-exposure chemical analysis for nicotine, were employed to detect/distinguish aerosol dilutions from a reference Kentucky 3R4F cigarette and two commercially available e-cigarettes (Vype eStick and ePen). ePen aerosol induced 97%, 94% and 70% less cytotoxicity than 3R4F cigarette smoke based on matched EC
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