Voltar
Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Fraction of <b><i>Auxemma oncocalyx</i></b> and Oncocalyxone A Affects the In Vitro Survival and Development of Caprine Preantral Follicles Enclosed in Ovarian Cortical Tissue

2016; Volume: 23; Issue: 5 Linguagem: Inglês

10.1159/000450719

ISSN

2504-2106

Autores

Johanna Leiva-Revilla, Laritza Ferreira de Lima, Simone Vieira Castro, C.C. Campello, Valdevane Rocha Araújo, Juliana Jales de Hollanda Celestino, Otília Deusdênia Loiola Pessoa, Edilberto R. Silveira, Ana Paula Ribeiro Rodrigues, J.R. Figueiredo,

Tópico(s)

Bioactive Compounds and Antitumor Agents

Resumo

<b><i>Background:</i></b><i>Auxemma oncocalyx</i> and its main component oncocalyxone A (onco A) have a high level of antioxidant and antitumor activity, but there are no studies on the action of both of these drugs regarding folliculogenesis. <b><i>Material and Methods:</i></b> Caprine ovarian tissue fragments were fixed (non-cultured control) or cultured for 1 or 7 days in α-MEM<sup>+</sup> alone (cultured control) or supplemented with dimethyl sulfoxide (DMSO; 20% v/v), bone morphogenetic protein 15 (BMP-15; 100 ng/ml), doxorubicin (DXR; 0.3 g/ml), or different concentrations of <i>A. oncocalyx</i> (1.2, 12, or 34 g/ml) or onco A (1, 10, or 30 g/ml). We analyzed for follicular morphology and growth, apoptosis (terminal deoxynucleotidyl transferase dUTP-biotin nick end labeling (TUNEL) assay), and cell proliferation (silver staining of argyrophilic nucleolus organizer regions (AgNOR) and test for proliferating cell nuclear antigen (PCNA)). <b><i>Results:</i></b><i>A. oncocalyx</i> and onco A (in a concentration-dependent manner) and DXR decreased (P < 0.05) the number of morphologically normal follicles, with no effect (P > 0.05) on follicular growth. <i>A. oncocalyx</i> reduced (P < 0.05) the percentage of normal follicles compared to onco A, whereas DXR, <i>A. oncocalyx</i> 1.2 g/ml, and onco A 1 g/ml increased (P < 0.05) the percentage of TUNEL-positive follicles. DXR decreased (P < 0.05) the number of nucleolus organizer regions. <b><i>Conclusion:</i></b><i>A. oncocalyx</i> and onco A affected the in vitro caprine folliculogenesis in a concentration-dependent manner. Onco A (1 g/ml) has a less harmful effect than DXR on goat preantral follicle survival.

Referência(s)