Human papillomavirus in non-genital Bowen's disease and bowenoid carcinoma
1994; John Libbey Eurotext; Volume: 4; Issue: 2 Linguagem: Inglês
ISSN
1952-4013
AutoresAldo Venuti, Pietro Donati, A. Amantea, L. Båluş,
Tópico(s)Cancer Diagnosis and Treatment
ResumoRecently the presence of HPV in non-genital Bowen's disease has been found in few cases. We analysed 13 cases of bowenoid carcinoma by the following technical aapproachs: I ― Southern-blot to define the physical state of viral sequences in the cells; II ― in situ hybridization to identify the single cell infected by specific HPVs; III ― PCR to detect even a very low level of virus sequences. By these methods we have found HPV 16 DNA sequences in 4 of 13 patients with different topographic presentations of Bowen's disease or bowenoid carcinoma. The sequences of HPV 16 DNA in all cases were in an unintegrated form but in a patient presenting two lesions located in the hand, finger and foot interdigital space, the latter one revealed the integration of viral sequences into the host genome
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