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Stable plastid transformation in Scoparia dulcis L.

2016; Springer Science+Business Media; Volume: 22; Issue: 4 Linguagem: Inglês

10.1007/s12298-016-0386-7

0971-5894

Muralikrishna Narra, Srinivas Kota, Kalva Bharath Kumar, Sadanandam Abbagani,

Plant biochemistry and biosynthesis

In the present investigation we report stable plastid transformation in Scoparia dulcis L., a versatile medicinal herb via particle gun method. The vector KNTc, harbouring aadA as a selectable marker and egfp as a reporter gene which were under the control of synthetic promoter pNG1014a, targets inverted repeats, trnR/trnN of the plastid genome. By use of this heterologous vector, recovery of transplastomic lines with suitable selection protocol have been successfully established with overall efficiency of two transgenic lines for 25 bombarded leaf explants. PCR and Southern blot analysis demonstrated stable integration of foreign gene into the target sequences. The results represent a significant advancement of the plastid transformation technology in medicinal plants, which relevantly implements a change over in enhancing and regulating of certain metabolic pathways.