Produção Nacional
Revisado por pares
First Report of Collar Rot on Jack Bean ( Canavalia ensiformis ) Caused by Sclerotium rolfsii in Brazil
2016; American Phytopathological Society; Volume: 101; Issue: 2 Linguagem: Inglês
10.1094/pdis-05-16-0622-pdn
ISSN1943-7692
AutoresJoão Victor Jansen de Queiroz, Eliane Mayumi Inokuti, Susan Satie Tsuji, Marcos Paz Saraiva Câmara, Sami Jorge Michereff,
Tópico(s)Plant Disease Management Techniques
ResumoHomePlant DiseaseVol. 101, No. 2First Report of Collar Rot on Jack Bean (Canavalia ensiformis) Caused by Sclerotium rolfsii in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Collar Rot on Jack Bean (Canavalia ensiformis) Caused by Sclerotium rolfsii in BrazilJ. V. J. Queiroz, E. M. Inokuti, S. S. Tsuji, M. P. S. Câmara, and S. J. MichereffJ. V. J. QueirozSearch for more papers by this author, E. M. InokutiSearch for more papers by this author, S. S. TsujiSearch for more papers by this author, M. P. S. CâmaraSearch for more papers by this author, and S. J. MichereffSearch for more papers by this authorAffiliationsAuthors and Affiliations J. V. J. Queiroz E. M. Inokuti S. S. Tsuji M. P. S. Câmara S. J. Michereff , Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil. Published Online:28 Nov 2016https://doi.org/10.1094/PDIS-05-16-0622-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Jack bean (Canavalia ensiformis L.) is a semierect annual legume, grown mainly as green manure and cover crop in soil erosion control programs. In May 2013, jack bean plants showing wilt, collar rot, white mycelial mats, and numerous brown sclerotia on the surface of the stem near the soil line were observed in a field located in Chã Grande, State of Pernambuco, Brazil. Of the entire planting, 25 to 30% showed symptoms and died. The sclerotia were collected and disinfested by immersion in 75% (v/v) ethanol for 2 min and in sterilized distilled water for 1 min. Sclerotia then were placed on potato dextrose agar (PDA) and incubated at 30°C. Numerous spherical sclerotia were formed on PDA after 15 days. The sclerotia (1 to 3 mm in diameter) were white at first and gradually turned dark brown. Aerial mycelia usually formed many narrow hyphal strands 4 to 9 µm wide. The white mycelium formed a typical clamp connection after 5 days. To fulfill Koch’s postulates, 10 healthy, 15-day-old jack bean seedlings were inoculated with sclerotia obtained from an 18-day-old culture. Dry sclerotia were placed directly on the base of the plant (three sclerotia per plant). Ten uninoculated jack bean seedlings were used as the control treatment. The inoculated and uninoculated plants were incubated in a humid growth at 28°C for 24 h and then maintained in a greenhouse. Five days after inoculation, the inoculated jack bean seedlings presented wilt and collar rot symptoms, whereas those not inoculated were symptomless. The fungus was reisolated from the artificially inoculated plants. To confirm the identity of the causal fungus, part of the elongation factor 1-alpha (EF1-α) gene and the complete internal transcribed spacer (ITS1-5.8S-ITS2 rRNA) region of the fungus were amplified using the primers EF595F (5′-CGTGACTTCATCAAG-AACATG) and EF1160R (5′-CCGATCTTGTAGACGTCCTG) (Wendland and Kothe 1997), and ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTT-ATTGATATGC-3′) (White et al. 1990), respectively. A DNA analysis showed 100% similarity to Athelia rolfsii (Curzi) Tu and Kimbr (teleomorph of Sclerotium rolfsii) for partial EF1-α gene (GenBank accession no. JF267798) and 99% to ITS region (DQ059578). The sequences were deposited in GenBank (EF1-α, KF850528; ITS, KT750883), and the culture was deposited in the Culture Collection of Phytopathogenic Fungi of the Universidade Federal Rural de Pernambuco (CMM 2897), Recife, Brazil. On the basis of symptoms, fungal colonies, the EF1-α and ITS sequences, and pathogenicity to the host, this fungus was identified as S. rolfsii Saccardo (Mordue 1974). The use of jack bean as green manure and cover crop represents a risk to the collar rot epidemics, mainly in bean fields, where contributes to increase or to maintain the inoculum of S. rolfsii in the soil (Paula Júnior et al. 2011). This disease has been observed on C. ensiformis in others countries (Riley 1960), however, in Brazil this is the first report.References:Mordue, J. E. M. 1974. CMI Descriptions of Pathogenic Fungi and Bacteria, No. 410. CMI, Kew, Surrey, U.K. Google ScholarPaula Júnior, T. J., et al. 2011. Summa Phytopathol. 37:218. https://doi.org/10.1590/S0100-54052011000400012 Crossref, Google ScholarRiley, E. A. 1960. Mycol. Pap. 75:1. Google ScholarWendland, J., and Kothe, E. 1997. Mycol. Res. 101:798. https://doi.org/10.1017/S0953756296003450 Crossref, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego. Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 2 February 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 13 Jan 2017Published: 28 Nov 2016First Look: 24 Oct 2016Accepted: 19 Oct 2016 Pages: 388-388 Information© 2017 The American Phytopathological SocietyCited byAthelia rolfsii (sclerotium rot)CABI Compendium, Vol. CABI CompendiumOccurrence of collar rot on berseem ( Trifolium alexandrinum L.) caused by Sclerotium rolfsii and its molecular characterization in India7 November 2021 | Archives of Phytopathology and Plant Protection, Vol. 55, No. 1First Report of Southern Blight on Helianthus tuberosus Caused by Athelia rolfsii in ChinaJ. Zhong, C. J. Zhang, J. Z. Zhu, and H. J. Zhu7 February 2019 | Plant Disease, Vol. 103, No. 4First Report of Stem and Root Rot of Aconitum carmichaelii Caused by Sclerotium rolfsii in ChinaL. H. Xue, Y. Liu, and C. J. Li27 October 2017 | Plant Disease, Vol. 102, No. 1
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