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BIBTEX RIS

Control of Listeria innocua biofilms by biocompatible photodynamic antifouling chitosan based materials

2016; Elsevier BV; Volume: 137; Linguagem: Inglês

10.1016/j.dyepig.2016.10.020

1873-3743

Kelly A. D. F. Castro, Nuno M. M. Moura, Ana M. Fernandes, M. Amparo F. Faustino, Mário M.Q. Simões, José A. S. Cavaleiro, Shirley Nakagaki, Adelaide Almeida, Ângela Cunha, Armando J. D. Silvestre, Carmen S. R. Freire, Ricardo J.B. Pinto, Maria G. P. M. S. Neves,

Marine Sponges and Natural Products

New materials obtained through the incorporation of meso-tetraarylporphyrins, bearing phenyl or pentafluorophenyl groups at the meso positions with or without acid groups, in chitosan films have been prepared and characterized in detail. The efficiency of these porphyrinic-chitosan films (PS-CF) to prevent Listeria innocua attachment and subsequent biofilm formation was evaluated under different irradiation protocols (dark, light, light + dark, dark + light). All tested porphyrinic-chitosan films were photostable and effective in reducing Listeria innocua attachment during a period of 24 h exposure to white light. Biofilm development was nearly completely inhibited in two of the PS-CF films (one containing the 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin and the other one of the thio-carboxylate porphyrins) after being exposed to white light for 24 h followed by incubation in the dark for 48 h. Experiments with the non-immobilized porphyrins against cell suspensions of the same Listeria innocua strain showed that, the photodynamic inactivation efficiency was dependent on porphyrin. The demonstrated antimicrobial activity of PS-CF films, associated with the easy recovery of these films, make them promising photodynamic antifouling materials.