Produção Nacional
Revisado por pares
First Report of Colletotrichum fructicola Causing Anthracnose on Annona Leaves in Brazil
2016; American Phytopathological Society; Volume: 101; Issue: 2 Linguagem: Inglês
10.1094/pdis-06-16-0927-pdn
ISSN1943-7692
AutoresJ. F. O. Costa, Roberto Ramos‐Sobrinho, Tamires Paixão Chaves, J. R. A. Silva, Danilo Batista Pinho, Iraíldes Pereira Assunção, Gaus Silvestre de Andrade Lima,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 101, No. 2First Report of Colletotrichum fructicola Causing Anthracnose on Annona Leaves in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum fructicola Causing Anthracnose on Annona Leaves in BrazilJ. F. O. Costa, R. Ramos-Sobrinho, T. P. Chaves, J. R. A. Silva, D. B. Pinho, I. P. Assunção, and G. S. A. LimaJ. F. O. Costa, R. Ramos-Sobrinho, T. P. Chaves, J. R. A. Silva, D. B. Pinho, I. P. Assunção, and G. S. A. LimaAffiliationsAuthors and Affiliations J. F. O. Costa R. Ramos-Sobrinho T. P. Chaves J. R. A. Silva , Setor de Fitossanidade/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000 D. B. Pinho , Departamento de Fitopatologia, Universidade de Brasília, Brazil, 70910-900 I. P. Assunção G. S. A. Lima , Setor de Fitossanidade/Centro de Ciências Agrárias, Universidade Federal de Alagoas, Rio Largo, AL, Brazil, 57100-000. Published Online:21 Nov 2016https://doi.org/10.1094/PDIS-06-16-0927-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Anthracnose, caused by different Colletotrichum species, is major disease on Annona spp. (Kamei et al. 2014). This disease has been mainly associated with the Colletotrichum gloeosporioides complex (Kamei et al. 2014). In June 2012, two typical isolates of the C. gloeosporioides complex were obtained from symptomatic leaves (dark, circular, and necrotic lesions) of plants located in the counties of Maragogi and Estrela de Alagoas, Alagoas state, Brazil. Single-conidium cultures were obtained on potato dextrose agar (PDA) for both isolates, and deposited at the Coleção de Culturas de Fungos Fitopatogênicos da Universidade Federal de Alagoas (codes COUFAL1111 and COUFAL7777). Colonies on PDA were light gray with whitish edges, and cottony. The underside of the colony was uniformly gray presenting sectors toward the edges with a growth rate of 9.0 mm/day. Conidia were cylindrical, aseptate, hyaline, rounded at both ends, and 10.5 to 22.5 µm long and 3 to 6.5 µm wide. The morphological characteristics were consistent with descriptions of C. gloeosporioides sensu lato (Weir et al. 2012). To confirm the identity, partial sequences of manganese-superoxide dismutase (SOD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), chitin synthase (CHS), calmodulin (CAL), β-tubulin 2 (TUB2), actin (ACT), and internal transcribed spacer (ITS) (accession nos. KX013517 to KX013530) were used in a search in the Q-bank fungi database and retrieved C. fructicola with 99 to 100% identities. In addition, Bayesian inference analysis using concatenated sequences (TUB2, ACT, CAL, CHS, GAPDH, ITS, and SOD) placed the Colletotrichum isolates reported here in a same clade with other C. fructicola specimens (study S19095 deposited in TreeBASE). To confirm pathogenicity, a conidial suspension (106 conidia/ml) in water was prepared from a 15-day-old culture (grown at 25°C) and sprayed on asymptomatic leaves of soursop (A. muricata L.) and sugar apple (A. squamosa L.). On control leaves, only sterilized water was used. Leaves were kept for 2 days in a humid chamber at 25°C. After 5 days, lesions were observed on 100% of the inoculated leaves. To fulfill Koch's postulates, the fungus C. fructicola was successfully reisolated from inoculated leaves. No disease symptoms were observed on the control leaves. Other Colletotrichum species were recorded on Annona spp. (Kamei et al. 2014), but no isolate was genetically similar to C. fructicola. This pathogen was previously reported only on apple, cassava, and mango in Brazil (Bragança et al. 2016; Lima et al. 2013; Velho et al. 2015). To our knowledge, this is the first report of C. fructicola causing anthracnose on leaves of Annona spp. in Brazil.References:Bragança, C. A. D., et al. 2016. Plant Dis. 100:857. https://doi.org/10.1094/PDIS-06-15-0645-PDN Link, ISI, Google ScholarKamei, S. H., et al. 2014. Fruticultura Bras. 36:209. https://doi.org/10.1590/S0100-29452014000500025 Crossref, ISI, Google ScholarLima, N. B., et al. 2013. Fungal Divers. 61:75. https://doi.org/10.1007/s13225-013-0237-6 Crossref, ISI, Google ScholarVelho, A. C., et al. 2015. Fungal Biol. 119:229. https://doi.org/10.1016/j.funbio.2014.12.009 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 2 February 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 13 Jan 2017Published: 21 Nov 2016First Look: 17 Oct 2016Accepted: 12 Oct 2016 Page: 386 Information© 2017 The American Phytopathological SocietyCited byColletotrichum fructicolaCABI Compendium, Vol. CABI CompendiumPest categorisation of Colletotrichum fructicolaEFSA Journal, Vol. 19, No. 8Characterization of fungal species associated with cladode brown spot on Nopalea cochenillifera in Brazil11 September 2019 | European Journal of Plant Pathology, Vol. 155, No. 4Characterization, Phylogenetic Analyses, and Pathogenicity of Colletotrichum Species on Morus alba in Sichuan Province, ChinaLonghai Xue, Lei Zhang, Xiao Xiang Yang, Xiaoqin Huang, Wenxian Wu, Xiquan Zhou, James F. White, Yong Liu, and Chunjie Li7 August 2019 | Plant Disease, Vol. 103, No. 10Diversity of pathogenic and endophytic Colletotrichum isolates from Licania tomentosa in Brazil30 May 2018 | Forest Pathology, Vol. 48, No. 6Identification of Colletotrichum species associated with brown spot of cactus prickly pear in Brazil15 March 2018 | Tropical Plant Pathology, Vol. 43, No. 3
Referência(s)