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First Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in Brazil

2016; American Phytopathological Society; Volume: 101; Issue: 2 Linguagem: Inglês

10.1094/pdis-08-16-1168-pdn

1943-7692

Alessandra Jackeline Guedes de Moraes, Elineide Barbosa de Souza, R. L. R. Mariano, Adriano Márcio Freire Silva, Nelson Bernardi Lima, Ana Rosa Peixoto, Marco Aurélio Siqueira da Gama,

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HomePlant DiseaseVol. 101, No. 2First Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in BrazilA. J. G. Moraes, E. B. Souza, R. L. R. Mariano, A. M. F. Silva, N. B. Lima, A. R. Peixoto, and M. A. S. GamaA. J. G. MoraesSearch for more papers by this author, E. B. SouzaSearch for more papers by this author, R. L. R. MarianoSearch for more papers by this author, A. M. F. SilvaSearch for more papers by this author, N. B. LimaSearch for more papers by this author, A. R. PeixotoSearch for more papers by this author, and M. A. S. GamaSearch for more papers by this authorAffiliationsAuthors and Affiliations A. J. G. Moraes E. B. Souza R. L. R. Mariano A. M. F. Silva N. B. Lima , Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil A. R. Peixoto , Universidade do Estado da Bahia, Juazeiro, 48900-000, Bahia, Brazil M. A. S. Gama , Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil. Published Online:21 Nov 2016https://doi.org/10.1094/PDIS-08-16-1168-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Over the last few years, the appreciation of zucchini (Cucurbita pepo) has been growing due to its nutritional value and contribution to human health. In Brazil, it is among the 10 highest vegetables in economic value and production. During 2015, high incidence (about 80%) of soft rot was observed in green and ripe fruits of zucchini (var. Caserta) in a plantation located in Chã Grande County (8.2542472°S, 35.495197°W), Pernambuco State, Brazil. Soft rot symptoms in zucchini were water soaking and macerating of fruit. Samples were collected from zucchini fields and bacteria were selectively isolated by transferring rot tissue to a healthy pepper fruit using a sterilized toothpick (Takatsu et al. 1981). After 24 h incubation in moist chamber, bacteria were directly transferred from lesions to petri dishes containing CPG medium (peptone-casamino acid-glucose). In this medium, young colonies (24 h) of pectobacteria showed “broken glass” aspect when observed in stereoscope under oblique lighting. Bacterial strains CRMP1 to CRMP7 were tested for pathogenicity in fruits of pepper and zucchini, and potato tubers, six of each host for each strain, by injection of 10 µl of cell suspension (107 CFU/ml). After 24 to 48 h incubation in a moist chamber at 28°C, typical symptoms of soft rot were observed and strains were reisolated successfully from symptomatic zucchini fruits to complete Koch’s postulates. The strains were stored at the Coleção de Culturas Rosa Mariano of the Laboratório de Fitobacteriologia at the Universidade Federal Rural de Pernambuco. The controls remained symptomless. The Biolog Gen III System identified all strains as belonging to the genus Pectobacterium. The molecular identification was performed from the sequencing of a 1,238-bp fragment of 16S rRNA (Darrasse et al. 1994). BLASTn analysis of 16S rDNA showed the sequences of strains CRMP1 to CRMP4 (GenBank KX096060 to 63) shared 99.9% similarity to strain LMG2417 (JN600324.1) of P. aroidearum and the sequences of strains CRMP5 to CRMP7 (KX096064 to 66) shared 99.9% similarity to strain 8 (JF926723.1) of P. carotovorum subsp. brasiliensis. Phylogenetic analysis of 16S rDNA performed by Bayesian inference analysis between the sequences of these seven strains and sequences of species and subspecies of Pectobacterium from GenBank grouped strains CRMP1 to CRMP4 with P. aroidearum (LMG2417) with a posterior probability of 100% and strains CRMP5 to CRMP7 with P. carotovorum subsp. brasiliensis (strain 8) with a posterior probability of 99%. ERIC and BOX-PCR analyzes performed with type strains of P. carotovorum subsp. carotovorum (IBSBF863), P. carotovorum subsp. brasiliensis (CRMP36), P. atrosepticum (IBSBF1819), and Dickeya chrysanthemi (IBSBF231) showed strains CRMP1 to CRMP4 formed a distinct group, while CRMP5 to CRMP7 grouped with P. carotovorum subsp. brasiliensis (CRMP36) at 70% similarity level. This report evidenced the expansion of the known host range for P. aroidearum (Nabhan et al. 2013) and P. carotovorum subsp. brasiliensis and sheds light on the diversity of pectobacteria in Brazil, which is little studied. To our knowledge, this is the first report of soft rot caused by P. aroidearum and P. carotovorum subsp. brasiliensis on zucchini in Brazil and also of P. aroidearum in Brazilian territory.References:Darrasse, A., et al. 1994. Appl. Environ. Microbiol. 60:1437. Crossref, ISI, Google ScholarNabhan, S., et al. 2013. Int. J. Syst. Evol. Microbiol. 63:2520. https://doi.org/10.1099/ijs.0.046011-0 Crossref, ISI, Google ScholarTakatsu, A., et al. 1981. Fitopatol. Bras. 6:550. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 2 February 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 13 Jan 2017Published: 21 Nov 2016First Look: 24 Oct 2016Accepted: 17 Oct 2016 Page: 379 Information© 2017 The American Phytopathological SocietyCited byOccurrence, identification, and host range of Pectobacterium brasiliense causing soft rot on seed potato tubers in Turkey20 October 2022 | Journal of Plant Diseases and Protection, Vol. 130, No. 1Pectobacterium spp. isolated from rotting carrots obtained from markets in Gampaha district, Sri Lanka exhibit the potential of having broad host ranges23 May 2022 | European Journal of Plant Pathology, Vol. 163, No. 4Analysis of the Taxonomy, Synteny, and Virulence Factors for Soft Rot Pathogen Pectobacterium aroidearum in Amorphophallus konjac Using Comparative Genomics13 July 2022 | Frontiers in Microbiology, Vol. 13Pectobacterium aroidearumCABI Compendium, Vol. CABI CompendiumPectobacterium carotovorumCABI Compendium, Vol. CABI CompendiumCharacterization of Pectobacterium brasiliense strains from potato and vegetables in Israel30 August 2021 | Plant Pathology, Vol. 70, No. 9Analysis of the Taxonomy and Pathogenic Factors of Pectobacterium aroidearum L6 Using Whole-Genome Sequencing and Comparative Genomics2 July 2021 | Frontiers in Microbiology, Vol. 12Pectobacterium brasiliense: Genomics, Host Range and Disease Management5 January 2021 | Microorganisms, Vol. 9, No. 1Diseases Caused by Pectobacterium and Dickeya Species Around the World5 January 2021First Report of Pectobacterium aroidearum Causing Soft Rot of Pepper (Capsicum annuum) Fruits in BrazilA. 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